@article{oai:obihiro.repo.nii.ac.jp:00004334, author = {Sanetomo, Rena and 實友, 玲奈 and Habe, Ippei and Hosaka, Kazuyoshi and 保坂, 和良}, issue = {1}, journal = {Molecular Breeding}, month = {Jan}, note = {application/pdf, Solanum pinnatisectum Dunal (2n = 2x = 24) is a tuber-bearing Mexican wild species that has an extremely high level of resistance to late blight (Phytophthora infestans). However, this species is distantly related and isolated from cultivated potato species by a strict sexual reproduction barrier. Previously, we successfully obtained triploid interspecific hybrids between chromosome-doubled S. pinnatisectum and a South American diploid clone carrying the S locus inhibitor gene (Sli). In this study, chromosome doubling of triploid hybrids was done to produce hexaploids used as the recurrent and donor parents in the successive backcrossing scheme. In a BC2 population consisting of 198 genotypes, P. infestans was inoculated. Ninety-six genotypes were resistant, and 102 were susceptible. The presence of the Rpi-blb3–specific marker band perfectly matched the resistance phenotype, verifying that the Rpi-blb3 gene controlled resistance. BC1 and BC2 progenies were relatively easy to reciprocally cross with cultivars. Therefore, the Rpi-blb3 gene derived from S. pinnatisectum could be used in practical breeding to confer a high level of late blight resistance to potato varieties with the aid of the gene-specific marker. © 2019, Springer Nature B.V.}, title = {Sexual introgression of the late blight resistance gene Rpi-blb3 from a Mexican wild diploid species Solanum pinnatisectum Dunal into potato varieties}, volume = {39}, year = {2019} }