@article{oai:obihiro.repo.nii.ac.jp:00004296, author = {Toyotome, Takahito and 豊留, 孝仁 and Hagiwara, Daisuke and Watanabe, Akira and Kamei, Katsuhiko}, issue = {8}, journal = {Medical mycology}, month = {Nov}, note = {application/pdf, We designed primers and cycling probes to detect the tandem repeat (TR) of cyp51A promoter in Aspergillus fumigatus. A control-probe was designed to anneal to the outside of the TR region, whereas a TR-probe was designed to anneal to the inside of the TR region. For amplification and probe-hydrolysis detection, the CycleavePCR system was used. Although the difference between Ct values of the wild-type genome for the control-probe and the TR-probe was around -0.1, the difference between Ct values of TR-harboring strains was around 0.7. These data indicate that this is a simple method to detect TR in azole-resistant A. fumigatus.}, pages = {1042--1044}, title = {A simple method to detect the tandem repeat of the cyp51A promoter in azole-resistant strains of Aspergillus fumigatus}, volume = {56}, year = {2018} }