@article{oai:obihiro.repo.nii.ac.jp:00004291,
 author = {Chaisowwong, Warangkhana and Kusumoto, Akiko and 楠本, 晃子 and Hashimoto, Miho and Harada, Toshihiko and Maklon, Khuanwalai and Kawamoto, Keiko and 川本, 恵子},
 issue = {1},
 journal = {Journal of Veterinary Medical Science},
 month = {Feb},
 note = {application/pdf, Campylobacter jejuni is the major cause of human gastroenteritis worldwide. Under stress conditions, C. jejuni can enter a viable but non-culturable (VBNC) state. We found that the C jejuni was able to enter a VBNC state by prolonged incubation at 4°C. The standard isolation methods using pre-enrichment steps in Bolton broth or Preston broth could not detect the VBNC cells in spiked chicken meat. The transcription levels of virulence-associated genes (flaA, flaB, cadF, ciaB, cdtA, cdtB and cdtC) were expressed in VBNC cells but in low levels. The VBNC cells retained the ability to invade Caco-2 human intestinal epithelial cells in vitro. In most cases, VBNC cells failed to resuscitate in Caco-2 cells, but in some experiments, they formed colonies after co-incubation with host cells. Collectively, C jejuni enters into a VBNC state at 4°C and the VBNC C jejuni remains virulent which may possibly lead to disease in humans. C jejuni in VBNC state is a potential concern for food safety. © 2012 The Japanese Society of Veterinary Science.},
 pages = {43--50},
 title = {Physiological characterization of campylobacter jejuni under cold stresses conditions: Its potential for public threat},
 volume = {74},
 year = {2012}
}