@article{oai:obihiro.repo.nii.ac.jp:00004253,
 author = {Bannai, Hiroshi and 坂内, 天 and Nishikawa, Yoshifumi and 西川, 義文 and Ibrahim, Hany M. and Yamada, Kyoko and Kawase, Osamu and Watanabe, Jun-ichi and Sugimoto, Chihiro and Xuan, Xuenan and 玄, 学南},
 issue = {9},
 journal = {Journal of Veterinary Medical Science},
 month = {},
 note = {application/pdf, We established a recombinant strain of Toxoplasma gondii that overexpressed programmed cell death 5 (TgPDCD5), in order to evaluate the role of endogenous TgPDCD5 in macrophage apoptosis during T. gondii infection. Immunofluorescence microscopy revealed that overproduced TgPDCD5 with a hemagglutinin tag was localized in the cytosol, which was consistent with the localization of endogenous TgPDCD5. The induced TgPDCD5-HA was recognized as an additional band by Western blot analysis, indicating successful overexpression of TgPDCD5. Secretion and release of TgPDCD5 by the parasite was also up-regulated in a time-dependent manner, which reflected its overproduction. Apoptosis due to parasite infection and interferon-gamma treatment was significantly upregulated by the overexpression of TgPDCD5. These results suggest that endogenous TgPDCD5 plays a role in macrophage apoptosis during T. gondii infection.},
 pages = {1183--1189},
 title = {Overproduction of the pro-apoptotic molecule, programmed cell death 5, in Toxoplasma gondii leads to increased apoptosis of host macrophages},
 volume = {71},
 year = {2009}
}