{"created":"2023-05-15T15:17:57.627452+00:00","id":4200,"links":{},"metadata":{"_buckets":{"deposit":"4dab7f02-bb87-4001-8bf7-88c2711c6932"},"_deposit":{"created_by":12,"id":"4200","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"4200"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00004200","sets":["5"]},"author_link":["5907","378","176"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"678","bibliographicPageStart":"675","bibliographicVolumeNumber":"67","bibliographic_titles":[{},{"bibliographic_title":"Parasitology International","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Dense granule protein 7 (NcGRA7) is a potent diagnostic antigen of Neospora caninum. Some studies have reported on the difficult expression, low yield, and variable degree of solubility of recombinant NcGRA7. We aimed to unravel the possible causes for these issues and tested NcGRA7 antigenicity in enzyme linked immunosorbent assays (ELISAs). The NcGRA7 coding sequence (217 amino acids) was split into five amino acid regions: NcGRA7m (27–217), NcGRA7m3 (27–160), NcGRA7m4 (27–135), NcGRA7m5 (161–190), and NcGRA7m6 (188–217). Three fragments, NcGRA7m, NcGRA7m3 and NcGRA7m4, exhibited high antigenic properties when tested against experimentally-infected mice and dog sera by ELISA. High levels of IgG2 antibodies against NcGRA7m3 were observed in field dog sera. In experimentally and naturally-infected cattle, the N. caninum-specific sera only reacted with NcGRA7m, indicating that this antigenic region differs among the three animal species. This study presents valuable information about the antigenic properties and topology of NcGRA7, and highlights its suitability for diagnostic purposes. © 2018 Elsevier B.V.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Ireland"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.parint.2018.06.012","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. http://creativecommons.org/lisenses/by-nc-nd/4.1/"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11112001","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13835769","subitem_source_identifier_type":"ISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Abdelbaky, Hanan H.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fereig, Ragab M.","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Nishikawa, Yoshifumi","creatorNameLang":"en"},{"creatorName":"西川, 義文","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-12-01"}],"displaytype":"detail","filename":"manuscript.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"full text","url":"https://obihiro.repo.nii.ac.jp/record/4200/files/manuscript.pdf"},"version_id":"04e04b4f-9f4f-485f-a17a-a4c4498b9787"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Antigenicity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cattle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dense granule protein 7","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dog","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neospora caninum","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of the antigenic region of Neospora caninum dense granule protein 7 using ELISA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of the antigenic region of Neospora caninum dense granule protein 7 using ELISA","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-01"},"publish_date":"2019-12-01","publish_status":"0","recid":"4200","relation_version_is_last":true,"title":["Identification of the antigenic region of Neospora caninum dense granule protein 7 using ELISA"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-28T03:13:27.373848+00:00"}