@article{oai:obihiro.repo.nii.ac.jp:00004154,
 author = {Jirapattharasate, Charoonluk and Moumouni, Paul Franck Adjou and Cao, Shinuo and Iguchi, Aiko and Liu, Mingming and Wang, Guanbo and Zhou, Mo and Vudriko, Patrick and Efstratiou, Artemis and Changbunjong, Tanasak and Sungpradit, Sivapong and Ratanakorn, Parntep and Moonarmart, Walasinee and Sedwisai, Poonyapat and Weluwanarak, Thekhawet and Wongsawang, Witsanu and Suzuki, Hiroshi and Xuan, Xuenan and 玄, 学南},
 issue = {2},
 journal = {Parasitology Research},
 month = {Feb},
 note = {application/pdf, Babesia spp., Theileria orientalis, and Anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. In this study, we used PCR to detect the presence of Babesia bovis, Babesia bigemina, and T. orientalis in 279 beef cattle from Western Thailand and A. marginale in 608 beef cattle from the north, northeastern, and western regions. The PCRs were performed using species-specific primers based on the B. bovis spherical body protein 2 (BboSBP2), B. bigemina rhoptry-associated protein 1a (BbiRAP-1a), T. orientalis major piroplasm surface protein (ToMPSP), and A. marginale major surface protein 4 (AmMSP4) genes. To determine the genetic diversity of the above parasites, amplicons of B. bovis and B. bigemina ITS1-5.8s rRNA gene-ITS2 regions (B. bovis ITS, B. bigemina ITS), ToMPSP, and AmMSP4 genes were sequenced for phylogenetic analysis. PCR results revealed that the prevalence of B. bovis, B. bigemina, T. orientalis, and A. marginale in the Western region was 11.1, 12.5, 7.8, and 39.1 %, respectively. Coinfections of two or three parasites were observed in 17.9 % of the animals sampled. The study revealed that the prevalence of A. marginale in the western region was higher than in the north and northeastern regions (7 %). Sequence analysis showed the BboSBP2 gene to be more conserved than B. bovis ITS in the different isolates and, similarly, the BbiRAP-1a was more conserved than B. bigemina ITS. In the phylogenetic analysis, T. orientalis MPSP sequences were classified into types 3, 5, and 7 as previously reported. A. marginale MSP4 gene sequences shared high identity and similarity with each other and clustered with isolates from other countries. This study provides information on the prevalence and genetic diversity of tick-borne pathogens in beef cattle and highlights the need for effective strategies to control these pathogens in Thailand.},
 pages = {751--762},
 title = {Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand},
 volume = {116},
 year = {2017}
}