@article{oai:obihiro.repo.nii.ac.jp:00004152,
 author = {Nakamura, Chinatsu and Hayashi, K. and Mohamad, A. T. and Masatani, T. and Zhang, G. and Jia, H. and Kumagai, A. and Zhang, H. and Zhou, J. and Nishikawa, Yoshifumi and 西川, 義文 and Sugimoto, C. and Xuan, Xuenan and 玄, 学南},
 issue = {1-2},
 journal = {Journal of Protozoology Research},
 month = {Dec},
 note = {application/pdf, Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band corresponding to a molecular mass of 80 kDa, which was similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/BgTRAP-infected RK13 cells. Moreover, mice inoculated with VV/BgTRAP produced antibody that specifically reacted with the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.},
 pages = {7--13},
 title = {Construction of a recombinant vaccinia virus expressing Babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice},
 volume = {23},
 year = {2013}
}