{"created":"2023-05-15T15:17:55.111431+00:00","id":4144,"links":{},"metadata":{"_buckets":{"deposit":"f95ae307-484c-4779-a5cf-ac9237a494bd"},"_deposit":{"created_by":12,"id":"4144","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"4144"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00004144","sets":["12:315"]},"author_link":[],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"12","bibliographicPageStart":"8","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"Journal of Protozoology Research","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In this study, potential of the Percoll density gradient centrifugation method for separation of bovine red bold cells (RBCs) infected with each merozoite type of Babesia ovata was examined. By the Percoll density gradient centrifugation, bovine RBCs infected with active forms of B. ovata merozoites were essentially collected from one of the layers. Moreover, this method made it possible to eliminate its crisis forms, a morphologically abnormal intraerythrocytic form, effectively. Moreover, no adverse effect on viability of B. ovata merozoites was found. Our results suggest that this method will be applicable to sample preparation for biological analyses which require active forms of B. ovata merozoites.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Research Center for Protozoan Diseases"}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"National Research Center for Protozoan Diseases","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824856","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09174427","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Moeko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hatta, Takeshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Maeda, Hiroki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tsubokawa, Daigo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Alim, M Abdul","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tsuji, Naotoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Inoue, Noboru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Suzuki, Hiroshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Umemiya-Shirafuji, Rika","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-15"}],"displaytype":"detail","filename":"s-1 JPR-2007-4.pdf","filesize":[{"value":"803.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"fulltext","url":"https://obihiro.repo.nii.ac.jp/record/4144/files/s-1 JPR-2007-4.pdf"},"version_id":"bce19f18-0e49-43cf-bfd3-b65940f2cd77"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Babesia ovata","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"in vitro culture","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Percoll density gradient centrifugation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Application of Percoll density gradient centrifugation for separation of Babesia ovatainfected erythrocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Application of Percoll density gradient centrifugation for separation of Babesia ovatainfected erythrocytes","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["315"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-02-15"},"publish_date":"2018-02-15","publish_status":"0","recid":"4144","relation_version_is_last":true,"title":["Application of Percoll density gradient centrifugation for separation of Babesia ovatainfected erythrocytes"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2023-08-21T00:01:47.485789+00:00"}