{"created":"2023-05-15T15:16:59.375983+00:00","id":2983,"links":{},"metadata":{"_buckets":{"deposit":"3d502724-e969-4fe7-8111-3350d93630c4"},"_deposit":{"created_by":12,"id":"2983","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2983"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00002983","sets":["242:243"]},"author_link":["291","168"],"item_6_alternative_title_1":{"attribute_name":"その他（別言語等）の研究課題名","attribute_value_mlt":[{"subitem_alternative_title":"Biological significance of milk oligosaccharides for neotite","subitem_alternative_title_language":"en"}]},"item_6_contributor_5":{"attribute_name":"研究分担者","attribute_value_mlt":[{"contributorAffiliations":[{"contributorAffiliationNameIdentifiers":[{"contributorAffiliationNameIdentifier":"","contributorAffiliationScheme":"ISNI","contributorAffiliationURI":"http://www.isni.org/isni/"}],"contributorAffiliationNames":[{"contributorAffiliationName":"","contributorAffiliationNameLang":"ja"}]}],"contributorNames":[{"contributorName":"Nakamura, Tadashi","lang":"en"},{"contributorName":"中村, 正","lang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{"nameIdentifier":"168","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070271749","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000070271749"},{"nameIdentifier":"70271749","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70271749"}]}]},"item_6_description_10":{"attribute_name":"研究代表者番号","attribute_value_mlt":[{"subitem_description":"80185082","subitem_description_type":"Other"}]},"item_6_description_11":{"attribute_name":"研究機関","attribute_value_mlt":[{"subitem_description":"帯広畜産大学","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"報告者らは、ハナグマ、ツキノワグマ、イヌ、フサオマキザル乳のオリゴ糖、ならびにウシ、ヒツジの初乳のシアリルオリゴ糖の構造研究を行った。その中で、ハナグマ、ツキノワグマの乳においてはラクト-スよりもオリゴ糖の方が多いことを見出した。とくにツキノワグマ乳ではラクト-スは痕跡量しか存在せず、5糖や3糖が優勢糖質であった。真獣類食肉目の属するこれらの種は新生仔を未熟な状態で出産する哺乳動物種であることから、ラクト-ス以外のミルクオリゴ糖には、未熟な新生仔の成長や体形成に対して役割をもっている可能性が示唆された。また、未熟な新生仔を産む哺乳動物種であるタマ-ワラビ-(有袋類)の乳から分離したシアリルオリゴ糖を、線維芽細胞の培養系に入れて培養したところ、細胞増殖促進効果が認められた。この結果も、ミルクオリゴ糖に乳仔の体形成に役割のある可能性を示唆している。また、イヌ乳に硫酸基をもつオリゴ糖が認められた。これまで硫酸基をもつミルクオリゴ糖が認められている種は、ラット、ヒトそれに報告者によるイヌであり、これらはいずれも新生仔期に目の開いていない種である。このことから、硫酸基をもつミルクオリゴ糖には、乳仔への硫酸基の運搬を通じて網膜の形成に働きのある可能性が示唆された。一方、ウシ、ヒツジのシアリルラクト-スには、シアル酸の1位カルボキシル基と他の水酸基との間でラクトン環を形成した誘導体が検出された。これにはインフルエンザウィルスが乳仔腸管へ付着するのを阻止する感染防御的な働きのある可能性が示唆された。このように本研究を通じてミルクオリゴ糖には乳仔の体形成や、ウィルスなどに対する感染防御的な働きのあることが示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Chemical structures of milk oligosaccharides of coati, Japanese black bear, dog and brown capuchin and sialyl coiostrum oligosaccharides of cow and sheep were determined Coati and Japanese blackbear milk contain more amounts of oligosaccharides than lactose. In Japanese black bear milk, a pentasaocharide and a saccharide are dominant, while lactose is contained in a trace amount. As bear and coati cubs are small and immature (altricial), these milk oligosaccharides have biological significance on the growth and body formation of immature youngs. Also, the sialyl oligosaccharides separated from the milk of tammar waliaby, whose youngs are extremely immature, were shown to have growth activity on Balb/c 3T3 fibloblastcell in this study, suggesting that the oligosaccharides have biological role on the growth of youngs body. lactose 3'-sulfate was identifiedin dog milk in this project. The presence of this compound offers an explanation for the simultaneous delivery of sulfate and calcium via the milk, two essential nutrients in early life. lactose sulfate should be hydrolyzed in the gut of the youngand absorbed as inorganic sulfate. Sialyllactose lactones, in which the carboxyl group of sialic acid are dehydrated with the OH group of galactose in the structures, were detected in ovine and bovine colostrum in this project. These lactones are suggested to inhibit the attachment of influenza virus to the gut of youngs. In this project, milk oligosaccharides are assumed to have biological roles on the growth of youngs and the protectionfor the attack by virus or bacteria.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"平成10年度科学研究費補助金(一般研究(C))研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_6_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"09660124","subitem_description_type":"Other"}]},"item_6_text_12":{"attribute_name":"助成元","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"科学研究費助成事業"}]},"item_6_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"4837.pdf","filesize":[{"value":"15.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"4837.pdf","url":"https://obihiro.repo.nii.ac.jp/record/2983/files/4837.pdf"},"version_id":"5a2b3379-b304-4e15-b20e-768ce80d5b5e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Urashima, Tadasu","creatorNameLang":"en"},{"creatorName":"浦島, 匡","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ミルクオリゴ糖の乳児に対する生理的意義の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ミルクオリゴ糖の乳児に対する生理的意義の解明","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"12","path":["243"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-03-03"},"publish_date":"2009-03-03","publish_status":"0","recid":"2983","relation_version_is_last":true,"title":["ミルクオリゴ糖の乳児に対する生理的意義の解明"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-05-20T01:09:34.923293+00:00"}