{"created":"2023-05-15T15:16:59.330978+00:00","id":2982,"links":{},"metadata":{"_buckets":{"deposit":"cbc104aa-04ef-4887-953c-a035fce50a25"},"_deposit":{"created_by":12,"id":"2982","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2982"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00002982","sets":["242:243"]},"author_link":["58","5331","164","340","198","361","258"],"item_6_alternative_title_1":{"attribute_name":"その他（別言語等）の研究課題名","attribute_value_mlt":[{"subitem_alternative_title":"Studies on newly synthesized peptides having parasitocidal activity, and their mechanisms on host immune repsponses.","subitem_alternative_title_language":"en"}]},"item_6_contributor_5":{"attribute_name":"研究分担者","attribute_value_mlt":[{"contributorNames":[{"contributorName":"小俣, 吉孝","lang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"198","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002585123","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002585123"},{"nameIdentifier":"10132987","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10132987"}]},{"contributorNames":[{"contributorName":"藤崎, 幸蔵","lang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"340","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006874417","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006874417"},{"nameIdentifier":"00292095","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00292095"}]},{"contributorAffiliations":[{"contributorAffiliationNameIdentifiers":[{"contributorAffiliationNameIdentifier":"","contributorAffiliationScheme":"ISNI","contributorAffiliationURI":"http://www.isni.org/isni/"}],"contributorAffiliationNames":[{"contributorAffiliationName":"","contributorAffiliationNameLang":"ja"}]}],"contributorNames":[{"contributorName":"Igarashi, Ikuo","lang":"en"},{"contributorName":"五十嵐, 郁男","lang":"ja"}],"familyNames":[{"familyName":"Igarashi","familyNameLang":"en"},{"familyName":"五十嵐","familyNameLang":"ja"}],"givenNames":[{"givenName":"Ikuo","givenNameLang":"en"},{"givenName":"郁男","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"58","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000003078567","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000003078567"},{"nameIdentifier":"80159582","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80159582"},{"nameIdentifier":"read0167555","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0167555"}]},{"contributorAffiliations":[{"contributorAffiliationNameIdentifiers":[{"contributorAffiliationNameIdentifier":"","contributorAffiliationScheme":"ISNI","contributorAffiliationURI":"http://www.isni.org/isni/"}],"contributorAffiliationNames":[{"contributorAffiliationName":"","contributorAffiliationNameLang":"ja"}]}],"contributorNames":[{"contributorName":"Nagasawa, Hideyuki","lang":"en"},{"contributorName":"長澤, 秀行","lang":"ja"}],"familyNames":[{"familyName":"Nagasawa","familyNameLang":"en"},{"familyName":"長澤","familyNameLang":"ja"}],"givenNames":[{"givenName":"Hideyuki","givenNameLang":"en"},{"givenName":"秀行","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"164","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60172524","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60172524"},{"nameIdentifier":"read0017304","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0017304"}]},{"contributorAffiliations":[{"contributorAffiliationNameIdentifiers":[{"contributorAffiliationNameIdentifier":"","contributorAffiliationScheme":"ISNI","contributorAffiliationURI":"http://www.isni.org/isni/"}],"contributorAffiliationNames":[{"contributorAffiliationName":"","contributorAffiliationNameLang":"ja"}]}],"contributorNames":[{"contributorName":"Suzuki, Naoyoshi","lang":"en"},{"contributorName":"鈴木, 直義","lang":"ja"}],"familyNames":[{"familyName":"Suzuki","familyNameLang":"en"},{"familyName":"鈴木","familyNameLang":"ja"}],"givenNames":[{"givenName":"Naoyoshi","givenNameLang":"en"},{"givenName":"直義","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"258","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005634551","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005634551"},{"nameIdentifier":"10003071","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10003071"}]},{"contributorNames":[{"contributorName":"豊田, 裕","lang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"361","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000090050418","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000090050418"},{"nameIdentifier":"90050418","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90050418"}]}]},"item_6_description_10":{"attribute_name":"研究代表者番号","attribute_value_mlt":[{"subitem_description":"10002263","subitem_description_type":"Other"}]},"item_6_description_11":{"attribute_name":"研究機関","attribute_value_mlt":[{"subitem_description":"帯広畜産大学","subitem_description_type":"Other"}]},"item_6_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"世界最大の感染症にも拘わらず、原虫病に対する治療・予防薬の開発は、原虫が真核生物であるため、困難を極めている。そこで、宿主動物に毒性が無く、原虫を直接殺滅しうる殺原虫物質の発見が世界中で望まれている。本研究チ-ムは、原虫病治療において、原虫を直接殺滅しうる毒性の極めて低いペプチドの合成に成功した。\n(1)本ペプチドには細胞内寄生原虫の細胞内侵入阻止および増殖抑制効果が観察されることをトキソプラズマおよびネオスポ-ラ原虫を用いて明らかにした。また、in vitro培養細胞を用いた実験から、本ペプチドが宿主細胞に対して極めて毒性の低いことを証明した。\n(2)マウスを用いた感染実験から、合成ペプチド投与による感染防御効果が認められた。原虫の感染経路および合成ペプチドの投与経路の検討から、本ペプチドの殺原虫作用は、原虫に対する直接障害性というよりは、むしろ宿主の免疫反応を増強することにより、感染抵抗性を賦与する可能性が示唆されたため、BRL(免疫調整物質)としての機能が有力視された。\n(3)免疫賦活作用の機序を解明することは、結果として他の感染症に対する防御反応の解明および治療法の確立に応用することができる。従って、バベシア、ネオスポ-ラ、トリパノソ-マ、クリプトスポリジウム原虫に対する感染防御効果が期待される。\n(4)原虫感染防御に係わる原虫分子を同定する目的で、原虫遺伝子を導入したトランスジェニックマウスの作成を試み、トキソプラズマ原虫主要抗原であるP30トランスジェニックマウスの作成に成功した。\n本研究における合成ペプチドの新規開発は、他に類例のない極めて画期的な免疫賦活物質として世界的に高い評価を受けた。また,原虫病に対する治療・予防薬については、非特異的なBRLが有力視されている。従って、本ペプチドは原虫感染症に対する広スペクトルなワクチン候補としての評価も高く、実用化へ向けての更なる研究推進が望まれる。今後、本ペプチドの実用化に際しては、生体内における作用機序についての詳細な分子免疫学的或いは発生工学的検討が必要である。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"(1) Studies on newly synthesized peptides\nNewly synthesized peptide (Obioactin to Obiopeptides) of the activity units which were isolated from cytokines as an immunoregulator were examined in the effect of Toxoplasma chronically infected animals. Mice chronically infected with Toxoplasma gondii were treated with cyclophosphamide (Cyp), Obiopeptide- 1 (Obio- 1) and/or anti-CD4 monoclonal antibody to determine the effect of these immunosuppressive agents on the cysts in the brain. In the brain of non-treate, and infected Cyp-Obi-1 treated mice, with hematoxyline-eosine staining or anti-Toxoplasma ABC labelling staining, large typically rounded tissue cysts were mostly detected, and in some regions dividing microcysts were also observed in this experimental studies. In contrast, brain tissue from Cyp only or anti-CD4 treated infected mice had multiple degenerated cysts of varied sizes in some brain regions, as well as clusters of microcysts, however, such change was more striking in the anti-CD4 treated mice. Infected mice treated with a combination of Cyp and Obio- I showed a significantly higher survival of 80% compared to 20% survival in mice treated with Cyp only. These results indicate that reactivation of rupture of tissue cysts in mice treated with Cyp, chronically infected with Toxoplasma, might be mainly mediated by CD4 positive cells rather than other CD8 and CD5 positive cells.\n(2) Production of transgenic mice carrying protozoan gene\nSAG-1 (p30), the major surface protein of Toxoplasma gondii, is considered as an important ligand in the process of host cell invasion. If this protein is produced by host cells and interfere with that of parasites, then the host will become resistant to parasite invasion. Or, it may become more susceptible to infection due to the lack of immune response against p30 antigen. To generate transgenic mice carrying p30 gene, a 3.3kb DNA fragments, containing p30 cDNA fused with CAG promoter, that has been shown to direct a ubiquitous expression of a reporter gene in transgenic mice, were microinjected into one of the pronuclei of one-cell embryos of C57BL/6J, BA LB/c or Fl (C57BL/6J x C3H/He) mice. The embryos were transferred to the oviducts of pseudopregnant ICR mice. In the first series of experiment using inbred strains, two p30-positive founders (male C57BL/61 and female BALB/c) were obtained among 159 mice that developed from the injected eggs. Furthermore, we have obtained several P30-founder mice expressing the gene product and transmitting the gene to the next generation, by using Fl (C57BL/6J x C3H/He) embryos. As far as we are aware, this is the first transgenic mice bearing a protozoan gene derived from T.gondii.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"平成7年度～平成10年度科学研究費補助金(基盤研究A)研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_6_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"07406014","subitem_description_type":"Other"}]},"item_6_text_12":{"attribute_name":"助成元","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"科学研究費助成事業"}]},"item_6_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"H11saitou.pdf","filesize":[{"value":"22.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"H11saitou.pdf","url":"https://obihiro.repo.nii.ac.jp/record/2982/files/H11saitou.pdf"},"version_id":"8c40bd9d-34c5-4252-a628-60b2a5a8e686"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"齊藤, 篤志","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"5331","nameIdentifierScheme":"WEKO"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"殺原虫作用ペプチドの新規開発と宿主作用機作に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"殺原虫作用ペプチドの新規開発と宿主作用機作に関する研究","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"12","path":["243"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-07-29"},"publish_date":"2008-07-29","publish_status":"0","recid":"2982","relation_version_is_last":true,"title":["殺原虫作用ペプチドの新規開発と宿主作用機作に関する研究"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-07-02T03:23:56.744499+00:00"}