{"created":"2023-05-15T15:16:59.170557+00:00","id":2979,"links":{},"metadata":{"_buckets":{"deposit":"b222c4ea-f376-4f6e-b0e3-6b026cb7dc93"},"_deposit":{"created_by":12,"id":"2979","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2979"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00002979","sets":["242:243"]},"author_link":["198","58","210","258"],"item_6_alternative_title_1":{"attribute_name":"その他（別言語等）の研究課題名","attribute_value_mlt":[{"subitem_alternative_title":"Establishment of continuous in vitro cultivation of bovine and equine Babesia parasites and it's application to diagnostic methods","subitem_alternative_title_language":"en"}]},"item_6_contributor_5":{"attribute_name":"研究分担者","attribute_value_mlt":[{"contributorNames":[{"contributorName":"小俣, 吉孝","lang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"198","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002585123","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002585123"},{"nameIdentifier":"10132987","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10132987"}]},{"contributorAffiliations":[{"contributorAffiliationNameIdentifiers":[{"contributorAffiliationNameIdentifier":"","contributorAffiliationScheme":"ISNI","contributorAffiliationURI":"http://www.isni.org/isni/"}],"contributorAffiliationNames":[{"contributorAffiliationName":"","contributorAffiliationNameLang":"ja"}]}],"contributorNames":[{"contributorName":"Saito, Atsushi","lang":"en"},{"contributorName":"齋藤, 篤志","lang":"ja"}],"familyNames":[{"familyName":"Saito","familyNameLang":"en"},{"familyName":"齋藤","familyNameLang":"ja"}],"givenNames":[{"givenName":"Atsushi","givenNameLang":"en"},{"givenName":"篤志","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"210","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004409900","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004409900"},{"nameIdentifier":"10002263","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10002263"},{"nameIdentifier":"read0167486","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0167486"}]},{"contributorAffiliations":[{"contributorAffiliationNameIdentifiers":[{"contributorAffiliationNameIdentifier":"","contributorAffiliationScheme":"ISNI","contributorAffiliationURI":"http://www.isni.org/isni/"}],"contributorAffiliationNames":[{"contributorAffiliationName":"","contributorAffiliationNameLang":"ja"}]}],"contributorNames":[{"contributorName":"Suzuki, Naoyoshi","lang":"en"},{"contributorName":"鈴木, 直義","lang":"ja"}],"familyNames":[{"familyName":"Suzuki","familyNameLang":"en"},{"familyName":"鈴木","familyNameLang":"ja"}],"givenNames":[{"givenName":"Naoyoshi","givenNameLang":"en"},{"givenName":"直義","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"258","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005634551","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005634551"},{"nameIdentifier":"10003071","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10003071"}]}]},"item_6_description_10":{"attribute_name":"研究代表者番号","attribute_value_mlt":[{"subitem_description":"80159582","subitem_description_type":"Other"}]},"item_6_description_11":{"attribute_name":"研究機関","attribute_value_mlt":[{"subitem_description":"帯広畜産大学","subitem_description_type":"Other"}]},"item_6_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"赤血球内寄生原虫であるバベシアは牛、馬などに感染し世界的に甚大な被害を与えている。しかしながら、これらの原虫の培養法が未だ確立されていないため、研究材料の確保が難しく、特異性や感度の高い診断法、および抗バベシア剤やワクチンの開発についての検討が遅れている。本研究では、ウシ及びウマのバベシア原虫のin vitro長期連続培養法を確立し、更に培養で得られた原虫を用いて診断法について検討を行った。ウシバベシア原虫B.ovataでは、199培養液(40%ウシ血清添加、pH7.0)、赤血球容積が10%、低酸素のガス(5%酸素5%炭酸ガス90%窒素)の培養条件下でB.ovataの増殖が認められた。2代以後は3-4日間の継代培養間隔で赤血球感染率が6-10%に達し長期連続培養が可能となった。また、数代継代後は5%CO2のガス条件下でも培養可能であることが判明した。B.equiでは、199培養液(ハイポキサンチン、40%ウマ血清添加、pH7.0)、赤血球容積5〜10%、低酸素のガス(2%酸素5%炭酸ガス93%窒素)の培養条件でB.equiの増殖が認められた。2代以後は5-7日間の継代培養間隔で赤血球感染率が2-3%に達し連続培養が可能となった。同様に、B.caballiではRPMI1640培養液(L-glutamin、40%ウマ血清添加、pH7.0)赤血球容積が5〜10%、低酸素のガス(2%酸素5%炭酸ガス93%窒素)の培養条件下でB.caballiの増殖が認められた。2代以後は5-7日間の継代培養間隔で赤血球感染率が2-3%に達し連続培養が可能となった。また、これら培養で得られた原虫を抗原として用いた間接蛍光抗体法による血清中の抗体調査を行ったところ、モンゴルでは80%以上の馬が感染している事が明らかとなり、日本のウマ血清においても抗体の存在が疑われる例が認められた。また、特異的抗原の精製を目的としてB.equi及びB.caballiに対するモノクローナル抗体を作製した、その中の一つのモノクローナル抗体はB.equiの19kDAの抗原を認識し、in vitroで50%の増殖を抑制する。今後の、これらの抗体を認識する抗原を用いた新しい診断法の開発が必要である。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Babesiosis in cattle and equine is endemic and causes enormous economic losses throughout most of the tropical and subtropical regions of the world. In this study, establishment of continuous cultivation of bovine and equine Babesia parasites were examined.Babesia ovata was initiated their multiplication within adult bovine RBC in Medium 199 supplemented with 40% adult bovine serum under a low oxygen atmosphere, 5% O2,5% CO2 and 90% N2.Babesia equi and B.caballi were continuously cultured in vitro in M199 and RPMI 1640 medium respectively, supplemented with 2 mM L-glutamine, 20 mM HEPES and 40% adult horse serum in a low oxygen atmosphere (2%O2,5%CO2 and 93% N2). All parasite growth was enhanced by transfer of cultures from a low oxygen to 5% CO2 in air.\nWith the successful establishment of in vitro cultures for B.equi and B.caballi, in vitro propagated B.equi and B.caballi infected erythrocytes were used as antigen in the IFAT.None showed a positive reaction of a 1 : 80 serum dilution In the serum samples in Japan However, 3 of the 19 trace or false positive ((]SY.+-。[)) cases were observed as pseudo-positive ((]SY.+-。[) -+) in a weak and sharp fluorescence around the parasite of B.equi and/or B.caballi. Antibody survey indicated that both B.equi and B.caballi are endemic in horses in Central Mongolia with 81.8% of horses being seropositive to both infections. Monoclonal antibodies (MAb) were produced against B.equi and B.caballi. One of MAbrecognized 19kDa antigen of B.equi and inhibited the multiplication of B.equi in vitro. This MAb is now being utiuzed to purify the corresponding antigen in order to test as antigen for ELISA.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"平成7年度科学研究費補助金一般研究(C)研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_6_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"06660397","subitem_description_type":"Other"}]},"item_6_text_12":{"attribute_name":"助成元","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"科学研究費助成事業"}]},"item_6_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"I090303-2.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"I090303-2.pdf","url":"https://obihiro.repo.nii.ac.jp/record/2979/files/I090303-2.pdf"},"version_id":"40849314-f4fa-43ad-9a70-8b43cde9e25a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Igarashi, Ikuo","creatorNameLang":"en"},{"creatorName":"五十嵐, 郁男","creatorNameLang":"ja"}],"familyNames":[{"familyName":"Igarashi","familyNameLang":"en"},{"familyName":"五十嵐","familyNameLang":"ja"}],"givenNames":[{"givenName":"Ikuo","givenNameLang":"en"},{"givenName":"郁男","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"58","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000003078567","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000003078567"},{"nameIdentifier":"80159582","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80159582"},{"nameIdentifier":"read0167555","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0167555"}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ウシ及びウマ・バベシア原虫 in vitro 長期培養法の確立とその応用に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ウシ及びウマ・バベシア原虫 in vitro 長期培養法の確立とその応用に関する研究","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"12","path":["243"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-03-03"},"publish_date":"2009-03-03","publish_status":"0","recid":"2979","relation_version_is_last":true,"title":["ウシ及びウマ・バベシア原虫 in vitro 長期培養法の確立とその応用に関する研究"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-07-02T03:23:49.085376+00:00"}