{"created":"2023-05-15T15:16:58.845960+00:00","id":2974,"links":{},"metadata":{"_buckets":{"deposit":"c99dc785-3afc-4ea6-bc45-272aee19a956"},"_deposit":{"created_by":12,"id":"2974","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2974"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00002974","sets":["242:243"]},"author_link":["198","210","221","258"],"item_6_alternative_title_1":{"attribute_name":"その他（別言語等）の研究課題名","attribute_value_mlt":[{"subitem_alternative_title":"Studies on no-specifical immunoregulatory effects to the host by the addministration of newly activated-peptides derived from Toxoplasma lysate substance","subitem_alternative_title_language":"en"}]},"item_6_contributor_5":{"attribute_name":"研究分担者","attribute_value_mlt":[{"contributorNames":[{"contributorName":"小俣, 吉孝","lang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"198","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002585123","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002585123"},{"nameIdentifier":"10132987","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10132987"}]},{"contributorAffiliations":[{"contributorAffiliationNameIdentifiers":[{"contributorAffiliationNameIdentifier":"","contributorAffiliationScheme":"ISNI","contributorAffiliationURI":"http://www.isni.org/isni/"}],"contributorAffiliationNames":[{"contributorAffiliationName":"","contributorAffiliationNameLang":"ja"}]}],"contributorNames":[{"contributorName":"Satho, Motoyoshi","lang":"en"},{"contributorName":"佐藤, 基佳","lang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{"nameIdentifier":"221","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000005106285","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000005106285"},{"nameIdentifier":"50003140","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50003140"},{"nameIdentifier":"read0167494","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0167494"}]},{"contributorAffiliations":[{"contributorAffiliationNameIdentifiers":[{"contributorAffiliationNameIdentifier":"","contributorAffiliationScheme":"ISNI","contributorAffiliationURI":"http://www.isni.org/isni/"}],"contributorAffiliationNames":[{"contributorAffiliationName":"","contributorAffiliationNameLang":"ja"}]}],"contributorNames":[{"contributorName":"Saito, Atsushi","lang":"en"},{"contributorName":"齋藤, 篤志","lang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{"nameIdentifier":"210","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004409900","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004409900"},{"nameIdentifier":"10002263","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10002263"},{"nameIdentifier":"read0167486","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0167486"}]}]},"item_6_description_10":{"attribute_name":"研究代表者番号","attribute_value_mlt":[{"subitem_description":"10003071","subitem_description_type":"Other"}]},"item_6_description_11":{"attribute_name":"研究機関","attribute_value_mlt":[{"subitem_description":"帯広畜産大学","subitem_description_type":"Other"}]},"item_6_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"トキソプラズマ(Toxoplasma gondii,TpRH strain)原虫成分の水溶解成分(TLA)の一部は強い非特異的免疫賦活作用を有することは既に世界で認知されている。まず、マウスにTLAを筋肉内に投与すると生体内T-およびB-リンパ球の増数およびNatural Killer(NK)細胞の増数が認められた。また、大型単核細胞数の増数が観察されたので、その起源を検討した。TLA感作マウス脾臓内単核細胞をTLAあるいはIL-2存在下で6日間連続培養し、NK細胞非感受性標的細胞(P-815腫瘍細胞)とNK細胞感受性標的細胞(YAC-1腫瘍細胞)の細胞障害性試験を試みた。その結果、TLA添加培養では培養後5-6日までP-815細胞およびYAC-1細胞障害性が共に経日的に増加した。IL-2添加培養ではP-815細胞を障害するが、YAC-1細胞を障害しなかった。また、細胞膜表面抗原ではThy-1(+)あるいはAsialoGM1(+)の大型キラー細胞、いわゆるLymphokine Activated Killer(LAK)細胞の誘導であることが判明した。自家発癌ラッテおよびマウスにTLAを投与すると明かに腫瘍内に大型LAKキラー細胞が強く誘導され、腫瘍増殖は抑制されていた。このLAK細胞誘導の作用機序を検討するとTLA感作リンパ球のみでは誘導されなく感作マクロファージの混在が必須であり、とくに両細胞の接触時に最も強く誘導されるこが実験的に証明された。さらに本研究では、応用拡大利用の基礎的検討として放牧牛に認められる牛ピロプラズマ病に対する非特異的免疫賦活効果について一部研究を行った。放牧前にTLAを2回あるいは3回筋肉内に投与した牛と無TLA投与牛を同時に放牧し、一定期間毎に赤血球内ピロプラズマ感染率と一般臨床症状を検査した。無TLA投与対象牛では、放牧後4週以後パラジテミアが増加し元気食欲が減弱し発育は減退した。一方、TLA投与牛ではパラジテミアの出現は有意に低く、かつ遅延し、非感染牛と同様に発育は良好であった。少なくとも、TLAは強い非特異的免疫賦活作用を動物体内に与えることが明かになったので、諸種キラー細胞誘導に伴う免疫賦活細胞物質の分離精製を試みた。TLAモノクローナル抗体の作出から、LAK細胞誘導の最も強いTLAmAb-H6Eをみつけた。TLAmAb-H6Eは一部のTLA成分と反応し、極めて特異性の高い物質であることが判明したが、未だ全ての構造式を決定するまでには至っていない。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"An imunomodulator, such as TLA (Toxoplasma lysate antigens) was tested in the experiments. Growth of the tumor autoinduced by 20-methylcholanthrene (MC) in rats and mice was inhibited after intramuscular injection of TLA.The antitumor activity of TLA was most obvious in the early stage of tumor growth. When TLA was administered to rats before the appearance of tumor, tumor formation was delayd slightly. According to the immunohistological examination of tumor tisue with anti-Thy-1 antibody, the rats treated with TLA showed large Thy-1 positive or Lymphokine-Activated Killer (LAK) cells, whereas the untreated rats indicated only a few small Thy-1 positive or NK cells. In a series of these studies, TLA induces NK cells, cytotoxic T-lymphocytes and LAK cells in animals after injection, and the induction of these killer cells needs to exist in combination with macrophages and lymphocytes in in vivo. An activity unit in TLA was isolated now as TLA-H6E,and the inhibitory activation to the tumor growth was significantly strong in comparison with others. Using the TLA-H6E monoclonal antibody, the structure of H6E is now making clear.\nNewly synthesized peptides of the activity units which were isolated from cytokines (lymphokines) as an immunoregulator, Obiopeptide, were examined in the effect of Toxoplasma chronically infected animals. Mice chronically infected with Toxoplasma were treated with Chclophosphamide (Cyp), Obiopeptide-1 (Obio-1) and/or anti-CD4 monoclonal antibody to determine the effect of these immunosuppressive agents on the cysts in the brain. In the brain of non-treated, and infected Cyp-Obi-1 treated mice, with HE staining or anti-Toxoplasma ABC labelling staining, large typically rounded tissue cysts were mostly detected, and in some regions dividing microcysts were also founded. In contrast, brain tissue from Cyp only or anti-CD4 treated infected mice had multiple degenerated cysts of varied sizes in some brain regions, as well as clusters of microcysts, however, such change was more striking in the anti-CD4 treated mice. Infected mice treated with a combination of Cyp and Obi-1 showed a significantly higher survival of 80% compared to 20% survival in mice treated with Cyp only. Percent neutrophilic leucocytes, monocytes and lymphocytes in mice treated with a combination of Obi-1 and anti-CD4, or Obi-1 and Cyp were higher compared to those groups treated with anti-CD4 antibody, or Cyp only. The increase in neutrophilic leucocyte and lymphocyte counts after a combined Cyp and Obi-1 treatment may, like wise, contribute to the induction of resistance in mice aginst Toxoplasma gondii. These results indidcate that reactivation of rupture of tissue cysts in mice treated with Cyp, chronicaly infected with Toxoplasma, might be mainly mediated by CD4 positive cells rather than other CD8, CD5 cells. Furthermore, the increase of neutrophilic leukocytes might contribute to the induction of the resistnce to Toxoplamsa in mice, after treatment with Obi-1 and Cyp in combination. However, these results seem to suggest that the reactivation or rupture of tissue cysts in mice chronically infected with Toxoplasma is not principally correlated with the death of Cyp treated mice.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"平成6年度科学研究費補助金(一般研究A)研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_6_description_9":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"03404013","subitem_description_type":"Other"}]},"item_6_text_12":{"attribute_name":"助成元","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"科学研究費助成事業"}]},"item_6_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"H7suzuki.pdf","filesize":[{"value":"32.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"H7suzuki.pdf","url":"https://obihiro.repo.nii.ac.jp/record/2974/files/H7suzuki.pdf"},"version_id":"aecdb174-76ea-46f9-959d-9fc981f2336e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cat","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Isopora felis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Toxoplasma gondii","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Suzuki, Naoyoshi","creatorNameLang":"en"},{"creatorName":"鈴木, 直義","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"新規の原虫由来活性ペプチドによる宿主の非特異的免疫賦活性用機序に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新規の原虫由来活性ペプチドによる宿主の非特異的免疫賦活性用機序に関する研究","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"12","path":["243"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-01-10"},"publish_date":"2008-01-10","publish_status":"0","recid":"2974","relation_version_is_last":true,"title":["新規の原虫由来活性ペプチドによる宿主の非特異的免疫賦活性用機序に関する研究"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-07-02T03:23:59.149956+00:00"}