{"created":"2023-05-15T15:14:50.670682+00:00","id":280,"links":{},"metadata":{"_buckets":{"deposit":"43772cbd-481b-4446-ba7a-6b7abc9ecd9e"},"_deposit":{"created_by":12,"id":"280","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"280"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00000280","sets":["5"]},"author_link":["132","745","4","151","387","744","168","291"],"item_2_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ウシ優性卵胞と閉鎖卵胞の卵胞液中におけるN-アセチルグルコサミニルトランスフェラーゼ I 活性の解析","subitem_alternative_title_language":"ja"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"320","bibliographicPageStart":"315","bibliographicVolumeNumber":"51","bibliographic_titles":[{"bibliographic_title":"Journal of Applied Glycoscience","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The activity of α3-D-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was detected in bovine follicular fluid (bFF). The GnT I activity in bFF had a pH optimum of 5.8 and an absolute requirement for either Co2+, Mn2+, or Mg2+, the activity being stimulated by these cations in the above order. The apparent Km value for α1-3α1-6 mannopentaose of GnT I in bFF was 2.17 mM. The substrate specificity for the GnT I activity decreased in the following order: α1-3α1-6 mannopentaose> α1-3α1-6 mannotriose>α1-3 mannobiose. The GnT I activity in bFF from large atretic follicles was significantly higher than in that from large dominant follicles. Moreover there was no significant difference between the GnT I activities in bFF from dominant follicles collected before and after surge of luteinizing hormone (LH surge). These data suggest that the GnT I activity in bFF may reflect functional changes in the microenvironment which lead to follicular atresia.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Applied Glycoscience"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.5458/jag.51.315","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Japanese Society of Applied Glycoscience","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11809133","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13447882","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nozaki, Hirofumi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Miyamoto, Akio","creatorNameLang":"en"},{"creatorName":"宮本, 明夫","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Hayashi, Ken-go","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Matsui, Motozumi","creatorNameLang":"en"},{"creatorName":"松井, 基純","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Yoshida, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tadashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"Arai, Ikichi","creatorNameLang":"en"},{"creatorName":"荒井, 威吉","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"Urashima, Tadasu","creatorNameLang":"en"},{"creatorName":"浦島, 匡","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"urashima51(4),315-320,2004.pdf","filesize":[{"value":"331.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"urashima51(4),315-320,2004.pdf","url":"https://obihiro.repo.nii.ac.jp/record/280/files/urashima51(4),315-320,2004.pdf"},"version_id":"802d6768-d68c-4659-9f88-503f0ddd3d67"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"N-acetylglucosaminyltransferase I","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"dominant follicle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"atretic follicle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"follicular fluid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bovine","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"N-Acetylglucosaminyltransferase I Activity in Bovine Ovarian Follicular Fluids from Dominant and Atretic Follicles","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"N-Acetylglucosaminyltransferase I Activity in Bovine Ovarian Follicular Fluids from Dominant and Atretic Follicles","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-10-30"},"publish_date":"2015-10-30","publish_status":"0","recid":"280","relation_version_is_last":true,"title":["N-Acetylglucosaminyltransferase I Activity in Bovine Ovarian Follicular Fluids from Dominant and Atretic Follicles"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-04-30T07:01:21.482812+00:00"}