@article{oai:obihiro.repo.nii.ac.jp:00000222,
 author = {仙田, 晶嗣 and 中村, 正 and Urashima, Tadasu and 浦島, 匡 and Arai, Ikichi and 荒井, 威吉},
 issue = {4},
 journal = {日本畜産学会報, Nihon Chikusan Gakkaiho},
 month = {},
 note = {application/pdf, 生乳中の酵母の動態を明らかにすることを目的として，帯広畜産大学と近郊の酪農家3戸で飼養している健康なホルスタイン種乳牛（体細胞数1×105cfu/ml以下）の乳頭表皮，分房乳，バルク乳およびローリー乳中の酵母数の月別変動について調べた．各試料は1999年7月から2000年6月までの1年間，それぞれ毎月1回ずつ採取した．生乳中の酵母の検出率と平均酵母数はそれぞれ順に，乳頭表皮では70.1％，52cfu/cm2，分房乳は47.9％，1.8cfu/ml，バルク乳は100％，28cfu/mlおよびローリー乳では100％，66cfu/mlであった．乳頭表皮および各種類の生乳中において90％以上を占める酵母数の水準は，乳頭表皮が150cfu/cm2以下（90.8％），分房乳が10cfu/ml以下（94.4％），バルク乳が100cfu/ml以下（97.8％）およびローリー乳が150cfu/ml以下（91.7％）であると考えられた．生乳中の酵母数は夏から秋期に高く，冬から春期に低くなる季節的な変動傾向が認められた．健康な乳牛の分房乳中では，主な乳房炎起因菌（C. parapsilosis, C. lambica, C. catenulata, C. rugosa）は夏期に検出されたが，冬から春期には検出されなかった．, The objective of this study was to investigate the seasonal incidences of yeast and the seasonal changes of yeast counts (cfu/ml) in a high quality raw milk produced by healthy Holstein cows (≤1×105 somatic cells/ml) bred in Obihiro University of Agriculture and Veterinary Medicine and in three local dairy farms near the University campus. The populations of yeasts in teat milk, bulk milk and lorry tank milk as well as in one of teat surfaces which were collected in each month from July, 1999 to June, 2000 were counted. The incidences of yeasts in teat milk, bulk milk, lorry tank milk, and in one of teat surfaces were 47.9%, 100%, 100% and 70.1%, respectively, whereas the mean of yeast counts in these samples were 1.8cfu/ml, 28cfu/ml, 66cfu/ml and 52cfu/cm2, respectively. The levels of yeast counts, which occupied over 90% of the cumulative frequency distributions of the incidence, were ≤10cfu/ml (94.4%) in teat milk, ≤100cfu/ml (97.8%) in bulk milk, ≤150cfu/ml (91.7%) in lorry tank milk, and ≤150cfu/ml (90.8%) in teat surfaces. It was recognized that the yeast counts in these raw milk samples and in teat surfaces overall increased from summer to autumn, and then decreased from winter to spring. It was found that the four major pathogenic yeast species concerning mastitis (C. parapsilosis, C. lambica, C. catenulata, C. rugosa) in teat milk of healthy Holstein cows were detected in summer, not in winter.},
 pages = {517--523},
 title = {生乳中の酵母検出率と酵母数の季節変動},
 volume = {74},
 year = {2003}
}