{"created":"2024-02-29T03:57:55.888847+00:00","id":2000127,"links":{},"metadata":{"_buckets":{"deposit":"4eac028a-ab10-4725-a450-838b3d3f7915"},"_deposit":{"created_by":12,"id":"2000127","owner":"12","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2000127"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:02000127","sets":["74:75:1709088742149"]},"author_link":["74","284","264","23"],"control_number":"2000127","item_5_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Pathological study of mouse mastitis model infected with four different Prototheca species","subitem_alternative_title_language":"en"}]},"item_5_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-11","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"10","bibliographicPageStart":"1","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"帯広畜産大学学術研究報告","bibliographic_titleLang":"ja"}]}]},"item_5_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Prototheca 属は、クロレラ科の１属であり、植物表面、動物の消化管内、土壌、湖沼、汚水中などの湿潤環境に広く分布している。Prototheca 属は牛乳房炎の原因菌でもあり、P. bovis, P. ciferrii, P. blaschkeae を原因する乳房炎では乳房の腫脹、硬結、乳汁中の著しい凝塊、乳量低下などの症状がみられる。1990 年代以降Prototheca 属による牛乳房炎の報告は増加傾向にあり、日本を含む世界各国で確認されている。本研究では、異なるPrototheca 属４種の病原性に関する知見を得るために、免疫不全モデルマウスや健常な授乳期マウスの乳腺組織に各Prototheca 属を実験的に感染させて病理学的比較検討を行った。P.ciferrii とP. bovis、P. blaschkeae、P. vistulensis を実験に用いた。まず、未経産のSCID マウスに尾静脈から接種することで、血流感染におけるPrototheca 属の病原性と各臓器への親和性を病理学的に検討した。次に、授乳期のBALB/c マウス乳腺にPrototheca 属菌を乳頭口接種し、乳房での感染成立および乳飲みマウスへの経口的感染の有無を病理学的に検討した。SCID マウスへの感染実験の結果、P. bovis のみがマウスヘ感染性を示し、脳と腎組織に病変を形成した。乳腺組織へのP. bovis の血行性移行は認められなかった。授乳期BALB/c マウスに対する乳頭口接種では、P. bovis を接種した乳腺で肉眼的に褪色、腫脹、硬結感が認められ、組織学的に乳腺上皮細胞の消失と乳腺腔内で著しい増殖がみられた。P. ciferrii およびP. blaschkeae 接種乳腺では、肉眼的に乳腺組織の褪色が認められ、組織学的には菌の軽度増殖を伴う巣状の炎症がみられた。P. bovis、P. ciferrii およびP. blaschkeae はBALB/c マウスの泌乳期乳腺へ上行感染し、乳腺炎を引き起こした。牛乳房炎の原因菌としての報告が少ないことから病原性が不明であったP. ciferrii とP. blaschkeae は、少なくともマウス泌乳期乳腺組織に中等度炎症を引き起こすことがわかった。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"The genus Prototheca is a member of the family Chlorellaceae, and is widely distributed in moist environments such as the surfaces of plants, the digestive tracts of animals, soil, lakes, and sewage. Prototheca spp. is known to be a causative agent of bovine mastitis. Bovine mastitis affected by P. bovis, P. ciferrii and P. blaschkeae reveals swelling of the breast, induration, secretion of milk containing white flakes, and reduction in milk production. Since the 1990s, bovine mastitis caused by Prototheca spp. have been increasing, and is distributed in worldwide, including Japan. We experimentally infected immunocompromised mice and the mammary gland of healthy lactating mice with each Prototheca spp. to obtain knowledge on the pathogenicity of four different Prototheca spp. In this study, we used four different Prototheca spp., P. ciferrii, P. bovis, P. blaschkeae and P. vistulensis. Initially, the pathogenicity and organ tropism of Prototheca spp. in bloodstream infection were pathologically investigated by inoculating the SCID mice via the tail vein. Next, we inoculated the mammary glands of lactating BALB/c mice with Prototheca spp.by the teat orifice and examined pathologically for the establishment of the infection in the udder and oral transmission to the suckling mice. As a result of the experimental infection to SCID mice, only P. bovis showed infectivity in mice, and the pathological changes were observed in the brain and kidney. No blood-borne transfer of P. bovis to mammary tissue was observed. The mammary gland of lactating BALB/c mice, inoculated with P. bovis showed macroscopically discoloration, swelling, and hardening. Histopathology revealed loss of mammary gland epithelial cells and marked proliferation of organisms in the acinus. Mammary glands inoculated with P. ciferrii and P. blaschkeae showed macroscopically discoloration of mammary tissue and histologically focal inflammatory cell accumulations with mild organism proliferation. P. bovis, P. ciferrii and P. blaschkeae infected ascendingly in the lactating mammary glands of BALB/c mice. P. ciferrii and P. blaschkeae, whose virulency were unknown due to the few case reports of bovine mastitis, were found to infect the mammary gland of mice.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24556/0002000127","subitem_identifier_reg_type":"JaLC"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"帯広畜産大学","subitem_publisher_language":"ja"}]},"item_5_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"帯広畜産大学","subitem_rights_language":"ja"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11877585","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13485261","subitem_source_identifier_type":"PISSN"}]},"item_5_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_language":"ja","subitem_subject_scheme":"NDC"}]},"item_5_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"清水, 貴瑛","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Kamikawa, Akihiro","creatorNameLang":"en"},{"creatorName":"上川, 昭博","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Toyotome, Takahito","creatorNameLang":"en"},{"creatorName":"豊留, 孝仁","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Takahashi, Eiji","creatorNameLang":"en"},{"creatorName":"高橋, 英二","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Furuoka, Hidefumi","creatorNameLang":"en"},{"creatorName":"古岡, 秀文","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-02-29"}],"filename":"01_44_shimizu.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://obihiro.repo.nii.ac.jp/record/2000127/files/01_44_shimizu.pdf"},"version_id":"01c31616-5e1d-4e84-9ab5-60628fbb218c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"プロトテカ症","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"牛乳房炎","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"マウス乳房炎モデル","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"感染実験","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Protothecosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bovine Mastitis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mouse Mastitis Model","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Experimental Infection","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Prototheca 属４種を用いたマウス乳房炎モデルの病理学的解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Prototheca 属４種を用いたマウス乳房炎モデルの病理学的解析","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"12","path":["1709088742149"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-02-29"},"publish_date":"2024-02-29","publish_status":"0","recid":"2000127","relation_version_is_last":true,"title":["Prototheca 属４種を用いたマウス乳房炎モデルの病理学的解析"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-29T05:48:49.295320+00:00"}