{"created":"2023-07-19T07:52:37.922920+00:00","id":2000027,"links":{},"metadata":{"_buckets":{"deposit":"711082f8-1231-490d-b07d-dae91966b3c9"},"_deposit":{"created_by":12,"id":"2000027","owner":"12","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2000027"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:02000027","sets":["5"]},"author_link":["2076","362","312","381","6585"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-09","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"255","bibliographic_titles":[{"bibliographic_title":"Molecular and Biochemical Parasitology","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Babesia bovis, an intraerythrocytic hemoprotozoan parasite, causes the most pathogenic form of bovine babesiosis, negatively impacting the cattle industry. Comprehensive knowledge of B. bovis biology is necessary for developing control methods. In cattle, B. bovis invades the red blood cells (RBCs) and reproduces asexually. Micronemal proteins, which bind to sialic acid of host cells via their microneme adhesive repeat (MAR) domains, are believed to play a key role in host cell invasion by apicomplexan parasites. In this study, we successfully deleted the region encoding MAR domain of the BBOV_III011730 by integrating a fusion gene of enhanced green fluorescent protein-blasticidin-S-deaminase into the genome of B. bovis. The transgenic B. bovis, lacking the MAR domain of the BBOV_III011730, invaded bovine RBCs in vitro and grew at rates similar to the parental line. In conclusion, our study revealed that the MAR domain is non-essential for the intraerythrocytic development of B. bovis in vitro.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_2_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"37315901","subitem_relation_type_select":"PMID"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.molbiopara.2023.111576","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01666851","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tuvshintulga, 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直明","creatorNameLang":"ja"}],"familyNames":[{"familyName":"Yokoyama","familyNameLang":"en"},{"familyName":"横山","familyNameLang":"ja"}],"givenNames":[{"givenName":"Naoaki","givenNameLang":"en"},{"givenName":"直明","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"312","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002627114","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002627114"},{"nameIdentifier":"80301802","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80301802"},{"nameIdentifier":"read0074761","nameIdentifierScheme":"researchmap","nameIdentifierURI":"https://researchmap.jp/read0074761"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-09-01"}],"filename":"yokoyama_255.pdf","filesize":[{"value":"342 KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"fulltext","url":"https://obihiro.repo.nii.ac.jp/record/2000027/files/yokoyama_255.pdf"},"version_id":"d1d2277d-ca53-409d-a0e7-e298e1fd274e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Babesia bovis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"blood stage","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"BBOV_III011730 gene,","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ene disruption","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nvasion","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"growth","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microneme adhesive repeat (MAR) domain","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Disruption of a DNA fragment that encodes the microneme adhesive repeat domain-containing region of the BBOV_III011730 does not affect the blood stage growth of Babesia bovis in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Disruption of a DNA fragment that encodes the microneme adhesive repeat domain-containing region of the BBOV_III011730 does not affect the blood stage growth of Babesia bovis in vitro","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-07-19"},"publish_date":"2023-07-19","publish_status":"0","recid":"2000027","relation_version_is_last":true,"title":["Disruption of a DNA fragment that encodes the microneme adhesive repeat domain-containing region of the BBOV_III011730 does not affect the blood stage growth of Babesia bovis in vitro"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-11-15T08:17:12.788026+00:00"}