{"created":"2023-05-15T15:16:05.543565+00:00","id":1840,"links":{},"metadata":{"_buckets":{"deposit":"7ba7a495-aa59-44f1-9ddf-23aea1ae9e97"},"_deposit":{"created_by":12,"id":"1840","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1840"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00001840","sets":["74:75:89"]},"author_link":["293","4136","211","270"],"item_5_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Polygalacturonase activity produced by alfalfa culture of the psychrotrophic facultative snow mold Sclerotinia trifoliorum","subitem_alternative_title_language":"en"}]},"item_5_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"21","bibliographicPageStart":"15","bibliographicVolumeNumber":"25","bibliographic_titles":[{"bibliographic_title":"帯広畜産大学学術研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Research bulletin of Obihiro University","bibliographic_titleLang":"en"}]}]},"item_5_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"低温性通性雪腐菌核病菌Sclerotinia trifoliorum の低温適応現象を解明するために, 5℃及び20℃で産生するポリガラクツロナーゼ(PGase) 活性の性質を調べた。S. trifoliorum のアルファルファ培地での培養は5℃では160日間(最適培養日数), 20℃では45日間(最適培養日数) 行った。5℃及び20℃培養粗抽出液のPGase 活性は, それぞれ30.8±0.4 U/mL (抽出体積462mL, Total activity 14 200U), 36.5±0.9 U/mL (抽出体積454mL, Total activity 16 600U) であり, 低温においてもPGase 産生量が著しく低下しないことがわかった。これら粗抽出液をBio-Gel P-6 DG ゲル濾過クロマトグラフィーによる脱塩操作後に, 5℃及び20℃培養脱塩画分のPGase 活性の性質を調べた。\n温度依存性については, 最適温度は5℃及び20℃培養PGase 活性ともに50℃であり, 50℃での活性に対する各温度での相対活性によって両者を較べると, それらの曲線は両者でほぼ一致した。温度安定性については, 残存活性で比較すると, 5℃培養PGase 活性は15℃～35℃において僅かであるが20℃培養のものにくらべて不安定性を示した。一方, 45～55℃では若干の安定性を示した。pH 依存性については, ５℃培養PGase 活性の最適pH は4.5であり, 20℃培養は4.0であった。従って, これらの結果は5℃及び20℃培養によって産生された各々のPGase アイソザイムが異なることを示した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"In order to clarify cold adaptation phenomenon of the psychrotrophic facultative snow mold Sclerotinia trifoliorum, the properties of polygalacturonase (PGase) activity produced in 5℃-and 20℃-culture were examined in the crude extracts. S. trifoliorum was cultured in the alfalfa medium at 5℃ for 160 days and at 20℃ for 45 days. The PGase activities in the crude extracts of the 5℃- and 20℃-culture were 30.8±0.4 U/mL (Extracted volume, 462 mL; Total activity, 14 200 U), 36.5±0.9U/mL (Extracted volume, 454 mL; Total activity, 16 600 U), respectively. It was shown that PGase production did not remarkably decrease in the low-temperature environment. After desalting of these crude extracts by the Bio-Gel P-6 DG gel filtration chromatography, the properties of PGase activities of the 5℃-and 20℃-culture desalting fractions were examined. As to the temperature dependence, the optimum temperature for the enzyme reaction at pH 4.5 was 50℃ for both 5℃-and 20℃-culture.\nBoth cultures were compared by the relative activity against the activity at 50℃, and both dependency-curves almost agreed within an experimental error. In the thermostability, compared with the 20℃-culture by remaining activity, the 5℃-culture PGase activity showed slightly more instability at 15℃-35℃, but a little more stability at 45℃-55℃. For the pH dependence, the optimum pH of 5℃-culture PGase activity was 4.5, and 20℃-culture was 4.0. Therefore, this fact shows that PGase isoenzymes produced by 5℃-and 20℃-culture were different from each other.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"帯広畜産大学","subitem_publisher_language":"ja"}]},"item_5_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"帯広畜産大学","subitem_rights_language":"ja"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11877585","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13485261","subitem_source_identifier_type":"PISSN"}]},"item_5_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡辺, 剛志","creatorNameLang":"ja"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"波川, 啓土","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"斉藤, 泉","creatorNameLang":"ja"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"高澤, 俊英","creatorNameLang":"ja"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"w061121-1.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"w061121-1.pdf","url":"https://obihiro.repo.nii.ac.jp/record/1840/files/w061121-1.pdf"},"version_id":"fccab564-a7d6-406c-95a0-600fa55a054b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Sclerotinia trifoliorum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"低温菌","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ポリガラクツロナーゼ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"低温適応","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞壁分解酵素","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Sclerotinia trifoliorum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"psychrotroph","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"polygalacturonase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cold adaptaition","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell wall degrading enzyme","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"低温性通性雪腐菌核病菌Sclerotinia trifoliorum のアルファルファ培養において産生されるポリガラクツロナーゼ活性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"低温性通性雪腐菌核病菌Sclerotinia trifoliorum のアルファルファ培養において産生されるポリガラクツロナーゼ活性","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"12","path":["89"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2006-11-21"},"publish_date":"2006-11-21","publish_status":"0","recid":"1840","relation_version_is_last":true,"title":["低温性通性雪腐菌核病菌Sclerotinia trifoliorum のアルファルファ培養において産生されるポリガラクツロナーゼ活性"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2023-11-14T07:22:46.217494+00:00"}