{"created":"2023-05-15T15:16:03.916848+00:00","id":1815,"links":{},"metadata":{"_buckets":{"deposit":"37a2598e-8376-4a8e-86b2-bd91d8dcf213"},"_deposit":{"created_by":12,"id":"1815","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1815"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00001815","sets":["74:75:86"]},"author_link":["53","73"],"item_5_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Magnetic particle-mediated gene transfer and magnetic selection of cells","subitem_alternative_title_language":"en"}]},"item_5_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"29","bibliographicPageStart":"23","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"帯広畜産大学学術研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Research bulletin of Obihiro University","bibliographic_titleLang":"en"}]}]},"item_5_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"微粒子銃装置（パーティクルガン）を用いて，磁性微粒子に遺伝子を担持させてタバコ（Nicotiana rustica）培養細胞やトウモロコシ（Zea mays L.），アスパラガス（Asparagus officinalis L.）およびカボチャ（Cucurbita pepo L.）の花粉に遺伝子を導入し磁力選抜を行った。磁性微粒子として、酸化鉄（マグネタイト，フェライト），鉄，ニッケル，鉄酸化物・金複合微粒子および金微粒子を用いた。一過的遺伝子の導入発現効率は，CaMV 35S プロモーターとNOS ターミネーターを組み込んだGUS 遺伝子を含むプラスミドｐBI221 用いて評価した。金微粒子を用いて遺伝子導入した時の一過的発現効率は低かったが，磁性微粒子を用いて磁気による細胞濃縮と磁力選抜を行うことによって遺伝子発現効率は顕著に増加した。例えば，トウモロコシ花粉へ磁性を有する酸化鉄微粒子（マグネタイト）を用いて撃ちこんだ時，GUS 遺伝子発現効率は8.5×10-4から2.1×10-2へと25 倍の向上があった。また，同様にカボチャ花粉の場合は6×10-4から2.3×10-2へと約38 倍の効果があったが、アスパラガス花粉についてはあまり大きな効果は認められなかった。これらの結果をもとに，除草剤biaraphos に抵抗性を有するbar 遺伝子を含むプラスミドpARK22 を磁性微粒子を用いてトウモロコシ、およびカボチャ花粉に撃ちこんだ。磁力選抜した花粉を受粉し，結実した種子を獲得した。その結果，トウモロコシの場合は5.3×10-3あるいは7.5×10-2の形質転換体作出効率が達成され，カボチャの場合は2.3×10-2の形質転換体作出効率が得られた。磁性微粒子を用いた新しいパーティクルガンプロセスは，磁力選抜により遺伝子導入細胞を高い効率で回収でき、また遺伝子導入花粉を受粉して簡便に形質転換体を作出する手法の発展に非常に有効であることが認められた。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_5_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"帯広畜産大学","subitem_publisher_language":"ja"}]},"item_5_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"帯広畜産大学","subitem_rights_language":"ja"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11877585","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13485261","subitem_source_identifier_type":"PISSN"}]},"item_5_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"615","subitem_subject_scheme":"NDC"}]},"item_5_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Kakuta, 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