@article{oai:obihiro.repo.nii.ac.jp:00001537,
 author = {Takashima, Yasuhiro and Xuan, Xuenan and 玄, 学南 and Nagasawa, Hideyuki and Matsumoto, Yasunobu and Igarashi, Ikuo and 五十嵐, 郁男 and Fujisaki, Kozo and Mikami, Takeshi and Otsuka, Haruki},
 issue = {4},
 journal = {The journal of protozoology research},
 month = {Oct},
 note = {application/pdf, To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the possibility of a PrV recombinant as a vaccine against cryptosporidiosis in animals.},
 pages = {173--179},
 title = {Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23},
 volume = {10},
 year = {2000}
}