{"created":"2023-05-15T15:15:48.579633+00:00","id":1488,"links":{},"metadata":{"_buckets":{"deposit":"3df463c4-5d56-4763-a947-e931b6c9c4bf"},"_deposit":{"created_by":12,"id":"1488","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1488"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00001488","sets":["12:25"]},"author_link":["339","3188","491","9","3185","3183","1048","295","324","3184","3187","176"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"41","bibliographicPageStart":"34","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"The journal of protozoology research","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were positive for antibodies to N. caninum. Of the 32 ELISA-positive samples, 29 (90.62%) were confirmed as positive by Western blot analysis with whole tachyzoite antigens. These results indicate that the recombinant NcGRA7t would be a reliable antigen in ELISA for the diagnosis of canine neosporosis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Research Center for Protozoan Diseases","subitem_publisher_language":"en"}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"National Research Center for Protozoan Diseases","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824856","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09174427","subitem_source_identifier_type":"PISSN"}]},"item_2_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"649","subitem_subject_scheme":"NDC"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hara, Olgga A","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Liao, Min","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Baticados, Waren","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"Bannai, Hiroshi","creatorNameLang":"en"},{"creatorName":"坂内, 天","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Zhang, Guohong","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zhang, Sofa","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lee, Eung-goo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Nishikawa, Yoshifumi","creatorNameLang":"en"},{"creatorName":"西川, 義文","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{},{}]},{"creatorNames":[{"creatorName":"Claveria, Florencia G.","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Igarashi, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Nagasawa, Hideyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"Xuan, Xuenan","creatorNameLang":"en"},{"creatorName":"玄, 学南","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"v16-3,4-1.pdf","filesize":[{"value":"98.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"v16-3,4-1.pdf","url":"https://obihiro.repo.nii.ac.jp/record/1488/files/v16-3,4-1.pdf"},"version_id":"a51ef17e-3edc-4d75-ad36-eaa7b8ce898f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Canine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ELISA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neospora caninum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NcGRA7","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["25"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-05-24"},"publish_date":"2007-05-24","publish_status":"0","recid":"1488","relation_version_is_last":true,"title":["Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-20T07:45:19.910319+00:00"}