{"created":"2023-05-15T15:14:44.799947+00:00","id":144,"links":{},"metadata":{"_buckets":{"deposit":"534e68cc-5b98-4c2f-9646-7633a0e2b87d"},"_deposit":{"created_by":12,"id":"144","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"144"},"status":"published"},"_oai":{"id":"oai:obihiro.repo.nii.ac.jp:00000144","sets":["5"]},"author_link":["569","168","570","4","291"],"item_2_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"市販発酵乳“フィルムヨーク”から分離したLeuconostoc mesenteroides subsp. cremorisの細胞壁多糖の化学的，免疫学的性質の解析","subitem_alternative_title_language":"ja"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"28","bibliographicPageStart":"21","bibliographicVolumeNumber":"49","bibliographic_titles":[{"bibliographic_title":"ミルクサイエンス","bibliographic_titleLang":"ja"},{"bibliographic_title":"Milk Science","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cell wall polysaccharides were separated from Leuconostoc mesenteroides subsp. cremoris isolated from “Filmjolk\"，a fermented milk made commercially in Sweden. Two Components(CNPI and CNP2) were further purified by gelfiltration on HPLC and the molecu1ar weights were  determined to be 850，000 and 81，000. CNP-2 was shown to contain two polysaccharides in  major and minor by an lH-NMR. The major polysaccharide was shown to have a  heptasaccharide repeating unit which contained five α and two β anomers，and also contained non reducing galactopyranose，O-2，3 disubstituted glucopyranose，O-3 substituted galactopyranose and O-3，6 disubstituted galactopyranose as units in  a molar ratio of 3:1:1:2. On the other hand，the minor polysaccharide was shown to have a tetrasaccharide repeating unit which contained three α and one β anomers， and also contained non reducing glucopyranose，O-3，6 disubstituted glucofuranose，O-4 substituted glucopyranose and O-6 substituted N-acetylglucosamine as units in amolar ratio of 1:1:1:1.Both CNP1 and CNP2 significantly stimulated the growth of mice spleen cells in vitro. ","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"スウェーデンで市販されている発酵乳\"Filmjolk\"から分離したLeuconostoc mesenteroides subsp.cremorisより，細胞壁多糖を分離した。同多糖はさらに， HPLCによるゲル濾過で2つの成分(CNP1とCNP2)に分離され，その分子量は850，000また81，000と推定された。CNP2は1H-NMR分析によって， 2種の多糖からなることが示された。そのうちの主要成分は， 5個のαアノマーおよび2個のβアノマーと，残基ユニットとして非還元末端ガラクトピラノース，2，3位置換ガラクトピラノース， 3位置換ガラクトピラノースおよび3，6位置換ガラクトピラノースを， 3:1:1:2のモル比で含むことが示された。一方少量成分は， 3個のα アノマーおよび1個のβアノマーと，残基ユニットとして非還元末端グルコピラノース，3，6位置換グルコフラノース， 4位置換グルコピラノースおよび6位置換N-アセチルグルコサミンを， 1:1:1:1のモル比で含むことが示された。CNP1，CNP2とも，試験管内でマウス脾臓細胞の増殖を有意に促進することが示された。","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Dairy Science Association","subitem_publisher_language":"en"}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Japanese Dairy Science Association","subitem_rights_language":"en"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11125739","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13430289","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mori, Fumiki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tadashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Nakamura, Osawa  Aya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"Arai, Ikichi","creatorNameLang":"en"},{"creatorName":"荒井, 威吉","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"Urashima, Tadasu","creatorNameLang":"en"},{"creatorName":"浦島, 匡","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-30"}],"displaytype":"detail","filename":"chemical.pdf","filesize":[{"value":"252.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"chemical.pdf","url":"https://obihiro.repo.nii.ac.jp/record/144/files/chemical.pdf"},"version_id":"8829e0b5-3944-4444-b256-672eee8001ed"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Chemical and Immunological Properties of Cell Wall Polysaccharide of Leuconostoc mesenteroides subsp. cremoris Isolated from the Commercial Fermented Milk，“Filmjolk\"","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Chemical and Immunological Properties of Cell Wall Polysaccharide of Leuconostoc mesenteroides subsp. cremoris Isolated from the Commercial Fermented Milk，“Filmjolk\"","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["5"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-05-23"},"publish_date":"2011-05-23","publish_status":"0","recid":"144","relation_version_is_last":true,"title":["Chemical and Immunological Properties of Cell Wall Polysaccharide of Leuconostoc mesenteroides subsp. cremoris Isolated from the Commercial Fermented Milk，“Filmjolk\""],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2024-02-28T03:07:55.638098+00:00"}