@article{oai:obihiro.repo.nii.ac.jp:00001280,
 author = {Tagawa, Michihito and Yamamoto, Yuhei and Shimbo, Genya and Iguchi, Aiko and Xuan, Xuenan and 玄, 学南 and Tomihari, Mizuki and Miyahara, Kazuro and 宮原, 和郎},
 issue = {5},
 journal = {Journal of Veterinary Medical Science},
 month = {May},
 note = {application/pdf, Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) was detected in normal human serum. We describe alternatively spliced mRNA expressed in peripheral blood mononuclear cells obtained from a healthy dog lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study.},
 pages = {871--875},
 title = {Gene and protein expression of a soluble form of CTLA-4 in a healthy dog},
 volume = {79},
 year = {2017}
}