@article{oai:obihiro.repo.nii.ac.jp:00001273,
 author = {Haneda, Shingo and 羽田, 真悟 and Nagaoka, Kentaro and Nambo, Yasuo and 南保, 泰雄 and Kikuchi, Masato and Nakano, Yasuko and Li, Junyou and Matsui, Motozumi and 松井, 基純 and Miyake, Yo-ichi and Imakawa, Kazuhiko},
 issue = {2},
 journal = {Journal of Reproduction and Development},
 month = {Apr},
 note = {application/pdf, From previous cDNA subtraction studies analyzing gene expression in equine endometrium, high lipocalin 2 (LCN2) mRNA expression was found in the gravid endometrium. In the uterus, LCN2 may transport hydrophobic molecules and siderophores with iron, or may form a complex with another protein, however, the expression of uterine LCN2 beyond day 20 of equine pregnancy and its receptor has not been characterized. To study the expression and potential roles of uterine LCN2 from pre-implantation to mid-gestation period, stage-specific endometrial samples were obtained from day 13 (day 0 = ovulation) cyclic and days 13, 19, 25, and 60 to 131 pregnant mares. Expression of LCN2 mRNA increased in day 19 gravid endometrium and was abundant from day 60 onward. The expression of LCN2 mRNA was localized to the glandular epithelium. LCN2 protein was detected in day 25 gravid endometrium and luminal fluid, and the protein was localized to the glandular epithelium and luminal cavity, whereas LCN2 receptor expression was found in luminal and glandular epithelium and trophectoderm throughout the experimental period. The presence of matrix metalloproteinase-9 (MMP9) was also examined because MMP9 is known to form a complex with LCN2. Although MMP9 and LCN2 were both found in luminal fluid from day 25 pregnant uterus, the complex of these proteins was not detected. Localization of the receptor in the trophectoderm suggests that endometrial LCN2 could play a role in carrying small substances from the mother to fetus in the equine species.},
 pages = {127--133},
 title = {Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares},
 volume = {63},
 year = {2017}
}