@article{oai:obihiro.repo.nii.ac.jp:00001070,
 author = {松嶋, 卯月 and 武田, 一夫 and Tanino, Karen K.},
 issue = {1},
 journal = {低温生物工学会誌},
 month = {},
 note = {application/pdf, In order to clarify the role of the vascular system for creating ice segregation, the water pathway through the stem of Keisukea japonica and the freezing front of ice segregation were investigated. Harvested stems of Keisukea japonica were used as plant samples. The water pathway was visualized with a 2.5mM fluorescein solution. After ice segregation was induced, the stem was tightly fixed on a microtome that cross sectioned the stem every 40μm. The light source with excitation filter illuminated the stem cross section. Fluorescence emission was captured by using CCD camera with fluorescent filter. Three-dimensional fluorescent image was reconstructed from the series of captured images. By observing horizontal cross sections, fluorescent illumination reached to the periphery of xylem. It means the freezing front should be the xylem surface. Pits on xylem cells, were 0.4μm of averaged short diameter. According to Ozawa (1989), ice segregation grew on the surface of a micropore membrane filter (≦0.2μm) that were placed in between supercooling water and surrounding atmosphere below 0℃. We anticipate the mechanism of ice segregation is based on the pore of the xylem pits and should be similar to the micropore membrane filter.},
 pages = {31--33},
 title = {シモバシラ(Keiskea japonica Miq.)氷晶形成における通水経路および氷成長面},
 volume = {60},
 year = {2014}
}