@article{oai:obihiro.repo.nii.ac.jp:00001034, author = {Ybañez, Adrian Patalinghug and Ybañez, Rochelle Haidee Daclan and Claveria, Florencia G. and Cruz-Flores, Mary Jane and Xuenan, Xuen and Yokoyama, Naoaki and 横山, 直明 and Inokuma, Hisashi and 猪熊, 壽}, issue = {7}, journal = {Journal of Veterinary Medical Science}, month = {}, note = {application/pdf, A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle.}, pages = {1009--1014}, title = {High Genetic Diversity of Anaplasma marginale Detected from Philippine Cattle}, volume = {64}, year = {2014} }