WEKO3
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First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines
https://obihiro.repo.nii.ac.jp/records/955
https://obihiro.repo.nii.ac.jp/records/955c35e838a-aeb8-4df9-8310-177f3f68b2a5
名前 / ファイル | ライセンス | アクション |
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inokuma75.27-36.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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公開日 | 2014-05-15 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Anaplasma marginale | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | cattle | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | PCR | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Philippines | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Rhipicephalus (Boophilus) microplus | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
Ybañez, Adrian Patalinghug
× Ybañez, Adrian Patalinghug× Sivakumar, Thillaiampalam× Ybañez, Rochelle Haidee Daclan× Ratilla, Jowarren Catingan× Perez, Zandro Obligado× Gabotero, Shirleny Reyes× Hakimi, Hassan× 河津, 信一郎× Matsumoto, Kotaro× 横山, 直明
WEKO
312
× 猪熊, 壽
WEKO
61
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抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α,gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale from the Philippines revealed 99.1–100% (16S rRNA, gltA, groEL and Msp5) and 94.3–97.6% (Msp1α) identities to the closest isolates from other countries. Moreover, sequence analysis of the Msp1α, gene showed 3 variants, including a case of co-infection with 2 variants. Phylogenetic analyses based on Msp1α and Msp5 genes revealed that Philippine A. marginale isolates formed a monophyletic lineage, and were phylogenetically related to Brazilian and Chinese isolates. On the other hand, a highly specific and sensitive nested PCR based on groEL, with a detection limit of 2 copies/PCR, was developed to detect A. marginale in the Philippines. This study reported the first molecular detection and characterization of A. marginale in cattle and R. microplus in Cebu, Philippines. | |||||||||||||
言語 | en | |||||||||||||
書誌情報 |
en : Journal of Veterinary Medical Science 巻 75, 号 1, p. 27-36, 発行日 2013 |
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出版者 | ||||||||||||||
言語 | en | |||||||||||||
出版者 | Japanese Society of Veterinary Science | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||
収録物識別子 | 09167250 | |||||||||||||
書誌レコードID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA10796138 | |||||||||||||
DOI | ||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||
識別子タイプ | DOI | |||||||||||||
関連識別子 | info:doi/10.1292/jvms.12-0268 | |||||||||||||
権利 | ||||||||||||||
言語 | en | |||||||||||||
権利情報 | Japanese Society of Veterinary Science | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | VoR | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |