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Sexing of Dog Sperm by Fluorescence In Situ Hybridization
https://obihiro.repo.nii.ac.jp/records/950
https://obihiro.repo.nii.ac.jp/records/95089db1d9e-b243-4eb8-b0da-ec8ca00c5852
名前 / ファイル | ライセンス | アクション |
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suzuki59.92-96.pdf (727.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-04-30 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Sexing of Dog Sperm by Fluorescence In Situ Hybridization | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Dog | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Fluorescence in situ hybridization (FISH) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sexing | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sperm | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Oi, Maya
× Oi, Maya× Yamada, Keisuke× Hayakawa, Hiroyuki× Suzuki, Hiroshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Effective preselection of sex has been accomplished in several species of livestock and also in humans using the flow cytometric sperm sorting method. A guaranteed high sorting accuracy is a key prerequisite for the widespread use of sperm sexing. The standard validation method is flow cytometric remeasurement of the DNA content of the sexed sperm. Since this method relies on the same instrument that produced the original sperm separation, it is not truly independent. Therefore, to be able to specifically produce either male or female offspring in the dog, we developed a method of direct visualization of sex chromosomes in a single sperm using fluorescence in situ hybridization (FISH) as a validation method. Denaturation of canine spermatozoa by immersion in 1 M NaOH for 4 min yielded consistent hybridization results with over 97% hybridization efficiency and a good preservation of sperm morphology. There was no significant difference between the theoretical ratio (50:50) and the observed ratio of X- and Y-chromosome-bearing spermatozoa in any of the three dogs. In addition, the mean purities of flow-sorted sex chromosomes in spermatozoa of the three dogs were 90.8% for the X chromosome fraction and 89.6% for the Y chromosome fraction. This sorting was evaluated by using the dual color FISH protocol. Therefore, our results demonstrated that the FISH protocol worked reliably for both unsorted and sexed sperm samples. | |||||
言語 | en | |||||
書誌情報 |
en : Journal of Reproduction and Development 巻 59, 号 1, p. 92-96, 発行日 2013 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Society for Reproduction and Development | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 09168818 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10936678 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1262/jrd.2012-098 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Reproduction and Development | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |