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Characterization of Toxoplasma gondii 5' UTR with Encyclopedic TSS Information
https://obihiro.repo.nii.ac.jp/records/882
https://obihiro.repo.nii.ac.jp/records/88213b3f054-a6bd-4b4d-9561-7ba4b46bf770
名前 / ファイル | ライセンス | アクション |
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445.pdf (215.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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公開日 | 2013-12-17 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Characterization of Toxoplasma gondii 5' UTR with Encyclopedic TSS Information | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Yamagishi, J.
× Yamagishi, J.× Watanabe, J.× Goo, Y. K.× Masatani, T.× Suzuki, Y.× 玄, 学南
WEKO
295
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | The 5′ UTR is widely involved in gene expression via post-transcriptional regulation. However, a detailed profile of the 5′ UTR for Toxoplasma gondii has not yet been demonstrated. To investigate the issue, we compared the predicted open reading frames (ORFs) and transcription start sites (TSSs) of T. gondii obtained by TSS-seq, a method that enables analysis of encyclopedic TSSs with next-generation sequencers. As a result, it was demonstrated that the mode length of the 5′ UTR is between 120 and 140 nucleotides (nts) when a subset of genes with predicted signal peptides was examined. However, when genes without the signal peptide were examined, the length was extended to approximately 600 nts. Because additional information on the predicted signal peptide generates increased reliability to the 5′ end estimation of each ORF, we believe that the former value was more reliable as a representative of the 5′ UTR length of T. gondii. The discrepancy suggests that current predictions of the 5′ end of the ORF were less accurate and considerably more discordant with the natural status. The 5′ untranslated region (5′ UTR) is defined as that between the 5′ end of the transcripts and just in front of a start codon of an ORF. Therefore, the 5′ UTR does not contain any information for a protein sequence; however, it is involved in the control of protein expression via the modulation of translational efficiency (Kozak, 1991b; Hughes, 2006). | |||||||||
言語 | en | |||||||||
書誌情報 |
en : Journal of Parasitology 巻 98, 号 2, p. 445-447, 発行日 2012 |
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出版者 | ||||||||||
出版者 | American Society of Parasitologists | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 00223395 | |||||||||
書誌レコードID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA00704304 | |||||||||
権利 | ||||||||||
権利情報 | American Society of Parasitologists | |||||||||
関連サイト | ||||||||||
識別子タイプ | URI | |||||||||
関連識別子 | http://www.journalofparasitology.org/doi/abs/10.1645/GE-2864.1 | |||||||||
関連名称 | http://www.journalofparasitology.org/doi/abs/10.1645/GE-2864.1 | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |