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Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay
名前 / ファイル | ライセンス | アクション |
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expression.pdf (503.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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公開日 | 2011-11-16 | |||||||||||||||
タイトル | ||||||||||||||||
言語 | en | |||||||||||||||
タイトル | Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay | |||||||||||||||
言語 | ||||||||||||||||
言語 | eng | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | Babesia gibsoni | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | diagnosis | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | enzyme-linked immunosorbent assay (ELISA) | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | thrombospondin-related adhesive protein (TRAP) | |||||||||||||||
資源タイプ | ||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
資源タイプ | journal article | |||||||||||||||
著者 |
Sandagdorj, Narantsatsral
× Sandagdorj, Narantsatsral× Goo, Youn-Kyoung× Terkawi, Mohamad Alaa
WEKO
359
× Soma, Takehisa× Luo, Yuzi× Li, Yan× Cao, Shinuo× Aboge, Gabriel Oluga× 西川, 義文
WEKO
176
× Badgar, Battsetseg× 玄, 学南
WEKO
295
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抄録 | ||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||
内容記述 | Among the previously established enzyme-linked immunosorbent assays (ELISAs), an ELISA using the full length of recombinant thrombospondin-related adhesive protein of Babesia gibsoni (rBgTRAPf) is considered as the most sensitive diagnostic method for the detection of antibody to B. gibsoni in dogs. However, the expression of rBgTRAPf in high concentration is poor and thus limits its usefulness as diagnostic antigen. To improve its expression level, we have truncated BgTRAPf into two fragments having either N- or C-terminus (BgTRAPn or BgTRAPc). The expression of BgTRAPc protein in Escherichia coli yielded adequate recombinant protein. The specificity and sensitivity of ELISAs with the truncated proteins were determined using B. gibsoni-experimentally infected dog sera and specific pathogen-free (SPF) dog sera. A total of 254 field dog sera were examined by the ELISA with rBgTRAPn, rBgTRAPc and rBgTRAPf as well as by indirect fluorescent antibody test (IFAT). The specificity of rBgTRAPc was highest (97.15%) and its kappa value was more (0.8003) than rBgTRAPn (0.7083). With sufficient level of expression as well as higher specificity and reliable sensitivity, rBgTRAPc appears to be a potential candidate antigen for serodiagnosis of B. gibsoni infection in dogs. | |||||||||||||||
言語 | en | |||||||||||||||
書誌情報 |
en : Experimental Parasitology 巻 129, 号 2, p. 196-202, 発行日 2011-10 |
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出版者 | ||||||||||||||||
言語 | en | |||||||||||||||
出版者 | Elsevier | |||||||||||||||
ISSN | ||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||
収録物識別子 | 00144894 | |||||||||||||||
書誌レコードID | ||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||
収録物識別子 | AA00641226 | |||||||||||||||
権利 | ||||||||||||||||
言語 | en | |||||||||||||||
権利情報 | Elsevier | |||||||||||||||
フォーマット | ||||||||||||||||
内容記述タイプ | Other | |||||||||||||||
内容記述 | application/pdf | |||||||||||||||
著者版フラグ | ||||||||||||||||
出版タイプ | VoR | |||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |