WEKO3
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A Study on Freeze-Drying as a Method of Preserving Mouse Sperm
https://obihiro.repo.nii.ac.jp/records/793
https://obihiro.repo.nii.ac.jp/records/793cbbaf71b-6da5-4318-95c4-b64d3edbfecb
名前 / ファイル | ライセンス | アクション |
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SRD.pdf (3.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-07-29 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A Study on Freeze-Drying as a Method of Preserving Mouse Sperm | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Comet assay | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Freeze-dried sperm | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | ICSI | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | SCSA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kawase, Yosuke
× Kawase, Yosuke× Suzuki, Hiroshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | This review describes the study of freeze-dried mouse sperm for practical application in preserving and transporting genetic resources. Freeze-dried sperm can be used to preserve and transport genetic resources; however, there still remain many areas which need to be studied. In particular, it is essential to assure long-term preservation over several decades or centuries. Recently, the theory of accelerated degradation kinetics to freeze-dried mouse sperm has been applied, and found that long-term preservation by conventional methods requires temperatures lower than -80 C. When the relationship between the pressure at primary drying and the preservation potential of freeze-dried mouse sperm was examined, a pressure of 0.37 mbar at primary drying significantly improved the developmental rate to the blastocyst stage. In addition, it has been shown that freeze-dried sperm stored at -80 C with and without transportation can retain their ability to generate viable offspring after storage for up to 2 years. Sperm chromatin structure assay (SCSA) was applied to mouse sperm freeze-dried under several conditions and compared the results with the embryonic developmental rates of freeze-dried sperm after intracytoplasmic sperm injection (ICSI) and with comet assay results. Furthermore, SCSA might be useful for estimation of developmental potential of fertilized eggs derived from ICSI using freeze-dried sperm in mice. | |||||
言語 | en | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | SRD Young Investigator Award | |||||
言語 | en | |||||
書誌情報 |
en : Journal of Reproduction and Development 巻 57, 号 2, p. 176-182, 発行日 2011 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Society for Reproduction and Development | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 09168818 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10936678 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1262/jrd.10-199E | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Japanese Society of Animal Reproduction | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |