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Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer
https://obihiro.repo.nii.ac.jp/records/703
https://obihiro.repo.nii.ac.jp/records/70313b49218-4f36-4335-9b9c-7bc676222d27
名前 / ファイル | ライセンス | アクション |
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production.pdf (1.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-02-03 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Interspecies | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Somatic cell nuclear transfer | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Whale | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Bhuiyan, Mohammad Musharraf Uddin
× Bhuiyan, Mohammad Musharraf Uddin× Suzuki, Yo× Watanabe, Hiroyuki× Lee, Eunsong× Hirayama, Hiroki× Matsuoka, Koji× Fujise, Yoshihiro× Ishikawa, Hajime× Ohsumi, Seiji× Fukui, Yutaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale (Balaenoptera borealis) interspecies somatic cell nuclear transfer (iSCNT) embryos. Moreover, trichostatin A (TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher (88.1%) in embryos reconstructed using the intracytoplasmic cell injection method (ICI) than that (48.7%) in the subzonal cell insertion (SUZI) counterpart. The rates of pseudopronucleus (PPN) formation (77.4 vs. 77.2%) and cleavage (24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were significantly (P<0.05) lower in the iSCNT embryos than in the parthenogenetic control (95.7% and 64.4%, respectively). Although 21.5% of the bovine parthenogenetic embryos developed to the blastocyst stage, no iSCNT embryo developed beyond the 6-cell stage. In Experiment 2, the cleavage rate did not vary between the TSA (50 nM)-treated and non-treated whale iSCNT embryos (30.5 vs. 32.3%, respectively). Moreover, it did not vary between the TSA-treated iSCNT and SCNT embryos (30.5 vs. 32.0%, respectively). Only one TSA non-treated iSCNT embryo developed to a compacted morula with 20 nuclei. One TSA-treated whale SCNT embryo developed to the 8-cell stage, and out of five whale iSCNT embryos, a 6-cell stage embryo was positive for whale DNA. In conclusion, bovine oocytes have the ability to support development of sei whale nuclei up to the 6-cell stage. | |||||
言語 | en | |||||
書誌情報 |
en : Journal of Reproduction and Development 巻 56, 号 1, p. 131-139, 発行日 2010 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Society for Reproduction and Development | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 09168818 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10936678 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1262/jrd.09-158A | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Japanese Society of Animal Reproduction | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |