WEKO3
アイテム
{"_buckets": {"deposit": "5ceeea31-cce1-4713-bce7-5354da56dfe9"}, "_deposit": {"created_by": 12, "id": "699", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "699"}, "status": "published"}, "_oai": {"id": "oai:obihiro.repo.nii.ac.jp:00000699", "sets": ["5"]}, "author_link": ["151", "278", "1507", "811", "1508"], "item_2_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "622", "bibliographicPageStart": "616", "bibliographicVolumeNumber": "56", "bibliographic_titles": [{"bibliographic_title": "Journal of Reproduction and Development", "bibliographic_titleLang": "en"}]}]}, "item_2_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Glucocorticoids modulate ovarian function in cattle. However, their regulatory mechanisms have not been fully elucidated. In the present study, we examined gene expression of two glucocorticoid-metabolizing enzymes, a bidirectional 11β-HSD type 1 (11HSD1) and a dehydrogenase 11β-HSD type 2 (11HSD2), and glucocorticoid receptor (GR) in bovine follicles during follicular maturation and atresia. Granulosa cells (GCs) and theca interna layers (TIs) were harvested from follicles classified as small growing, dominant, preovulatory, early atretic and late atretic follicles. The expression levels of 11HSD1, 11HSD2 and GR mRNA were quantified by real-time PCR. In the healthy follicles, expression of 11HSD1 mRNA increased as follicles matured, both in GCs and TIs. A significant negative correlation was found between the concentration of cortisol in follicular fluid and the level of 11HSD1 mRNA in GCs. The expression of 11HSD2 and GR was either very low or largely unchanged during follicular maturation. In the atretic follicles, a drastic increase in the expression of 11HSD2 was observed both in GCs and TIs. To assess the effect of FSH on the expression of 11HSDs and GR, GCs were cultured with FSH (0-100 ng/ml) for up to 6 days. FSH increased 11HSD1 mRNA in a dose-dependent manner, but not 11HSD2, nor GR. Taken together, these results suggest that developmentally-regulated 11HSD1 plays a pivotal role in modulating the local glucocorticoid environment in maturing bovine follicles.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_2_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Society for Reproduction and Development", "subitem_publisher_language": "en"}]}, "item_2_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1262/jrd.10-019K", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Japanese Society of Animal Reproduction", "subitem_rights_language": "en"}]}, "item_2_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10936678", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "09168818", "subitem_source_identifier_type": "PISSN"}]}, "item_2_text_25": {"attribute_name": "文献提供者", "attribute_value_mlt": [{"subitem_text_value": "手塚雅文(mtetsuka@obihiro.ac.jp)"}]}, "item_2_textarea_24": {"attribute_name": "作業記録", "attribute_value_mlt": [{"subitem_textarea_value": "Submitted by リポジトリ管理者 repo (repo@obihiro.ac.jp) on 2011-02-03T02:07:10Z\nNo. of bitstreams: 1\ngene.pdf: 381261 bytes, checksum: ab240b96d3224e7aecf8a980bc5b458a (MD5)"}, {"subitem_textarea_value": "Made available in DSpace on 2011-02-03T02:07:10Z (GMT). No. of bitstreams: 1\ngene.pdf: 381261 bytes, checksum: ab240b96d3224e7aecf8a980bc5b458a (MD5)\n Previous issue date: 2010"}]}, "item_2_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "Tetsuka, Masafumi", "creatorNameLang": "en"}, {"creatorName": "手塚, 雅文", "creatorNameLang": "ja"}], "familyNames": [{"familyName": "Tetsuka", "familyNameLang": "en"}, {"familyName": "手塚", "familyNameLang": "ja"}], "givenNames": [{"givenName": "Masafumi", "givenNameLang": "en"}, {"givenName": "雅文", "givenNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "278", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006400922", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006400922"}, {"nameIdentifier": "40311526", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40311526"}, {"nameIdentifier": "read0192793", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0192793"}, {"nameIdentifier": "0000-0001-7898-0414", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "https://orcid.org/0000-0001-7898-0414"}]}, {"creatorNames": [{"creatorName": "Nishimoto, Hiromi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "811", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miyamoto, Akio", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "151", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000010192767", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000010192767"}, {"nameIdentifier": "10192767", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=10192767"}, {"nameIdentifier": "read0167498", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0167498"}]}, {"creatorNames": [{"creatorName": "Okuda, Kiyoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1507", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000040177168", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000040177168"}]}, {"creatorNames": [{"creatorName": "Hamano, Seizo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1508", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-01-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "gene.pdf", "filesize": [{"value": "381.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 381300.0, "url": {"label": "gene.pdf", "url": "https://obihiro.repo.nii.ac.jp/record/699/files/gene.pdf"}, "version_id": "971e5cec-7dbd-4745-b479-0f87d6f60acd"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Bovine", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cortisol", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "11β-HSD", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Follicle", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Glucocorticoid receptor", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mRNA", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "12", "path": ["5"], "permalink_uri": "https://obihiro.repo.nii.ac.jp/records/699", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2011-02-03"}, "publish_date": "2011-02-03", "publish_status": "0", "recid": "699", "relation": {}, "relation_version_is_last": true, "title": ["Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone"], "weko_shared_id": -1}
Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone
https://obihiro.repo.nii.ac.jp/records/699
https://obihiro.repo.nii.ac.jp/records/699c8c86bef-1d2e-4789-b469-8697d3b9f4cf
名前 / ファイル | ライセンス | アクション |
---|---|---|
gene.pdf (381.3 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2011-02-03 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Bovine | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Cortisol | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | 11β-HSD | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Follicle | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Glucocorticoid receptor | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | mRNA | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | journal article | |||||||||||
著者 |
手塚, 雅文
× 手塚, 雅文
WEKO
278
× Nishimoto, Hiromi× Miyamoto, Akio
WEKO
151
× Okuda, Kiyoshi× Hamano, Seizo |
|||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | Glucocorticoids modulate ovarian function in cattle. However, their regulatory mechanisms have not been fully elucidated. In the present study, we examined gene expression of two glucocorticoid-metabolizing enzymes, a bidirectional 11β-HSD type 1 (11HSD1) and a dehydrogenase 11β-HSD type 2 (11HSD2), and glucocorticoid receptor (GR) in bovine follicles during follicular maturation and atresia. Granulosa cells (GCs) and theca interna layers (TIs) were harvested from follicles classified as small growing, dominant, preovulatory, early atretic and late atretic follicles. The expression levels of 11HSD1, 11HSD2 and GR mRNA were quantified by real-time PCR. In the healthy follicles, expression of 11HSD1 mRNA increased as follicles matured, both in GCs and TIs. A significant negative correlation was found between the concentration of cortisol in follicular fluid and the level of 11HSD1 mRNA in GCs. The expression of 11HSD2 and GR was either very low or largely unchanged during follicular maturation. In the atretic follicles, a drastic increase in the expression of 11HSD2 was observed both in GCs and TIs. To assess the effect of FSH on the expression of 11HSDs and GR, GCs were cultured with FSH (0-100 ng/ml) for up to 6 days. FSH increased 11HSD1 mRNA in a dose-dependent manner, but not 11HSD2, nor GR. Taken together, these results suggest that developmentally-regulated 11HSD1 plays a pivotal role in modulating the local glucocorticoid environment in maturing bovine follicles. | |||||||||||
言語 | en | |||||||||||
書誌情報 |
en : Journal of Reproduction and Development 巻 56, 号 6, p. 616-622, 発行日 2010 |
|||||||||||
出版者 | ||||||||||||
言語 | en | |||||||||||
出版者 | Society for Reproduction and Development | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | PISSN | |||||||||||
収録物識別子 | 09168818 | |||||||||||
書誌レコードID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AA10936678 | |||||||||||
DOI | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | info:doi/10.1262/jrd.10-019K | |||||||||||
権利 | ||||||||||||
言語 | en | |||||||||||
権利情報 | Japanese Society of Animal Reproduction | |||||||||||
フォーマット | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | application/pdf | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |