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Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates
https://obihiro.repo.nii.ac.jp/records/673
https://obihiro.repo.nii.ac.jp/records/6734b714fcd-e74e-434a-bdae-ed7d5e33c0f1
名前 / ファイル | ライセンス | アクション |
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Inoue073.pdf (84.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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公開日 | 2010-10-29 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | diagnosis | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | DNA template | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | LAMP | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | parasitic disease | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Trypanosoma | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | journal article | |||||||||||
著者 |
Thekisoe, Oriel M. M
× Thekisoe, Oriel M. M× Bazie, Raoul S. B× Coronel-Servian, Andrea M× Sugimoto, Chihiro
WEKO
252
× 河津, 信一郎× 井上, 昇
WEKO
63
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抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P>0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored from 1 day to 30 days. LAMP detection sensitivity was poor when fresh blood as DNA template was added directly into reactive solution. Results of this study demonstrated that LAMP has the potential to be used in field conditions for diagnosis of trypanosome infections without being affected by ambient temperatures of tropical and sub-tropical countries where trypanosomosis is endemic. | |||||||||||
言語 | en | |||||||||||
書誌情報 |
en : Journal of Veterinary Medical Science 巻 71, 号 4, p. 471-475, 発行日 2009-04 |
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出版者 | ||||||||||||
言語 | en | |||||||||||
出版者 | Japanese Society of Veterinary Science | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | PISSN | |||||||||||
収録物識別子 | 09167250 | |||||||||||
書誌レコードID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AA10796138 | |||||||||||
権利 | ||||||||||||
権利情報 | Japanese Society of Veterinary Science | |||||||||||
フォーマット | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | application/pdf | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |