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Establishment of an in vitro Transgene Expression System in Epimastigotes of Trypanosoma congolense
https://obihiro.repo.nii.ac.jp/records/671
https://obihiro.repo.nii.ac.jp/records/671264c60a7-e7d0-4410-90fa-ce120db75fe1
名前 / ファイル | ライセンス | アクション |
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establishment.pdf (168.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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公開日 | 2010-10-22 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Establishment of an in vitro Transgene Expression System in Epimastigotes of Trypanosoma congolense | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Trypanosoma congolense | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Epimastigotes | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | In vitro metacyclogenesis | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Transfection | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Transgene expression | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Sakurai, Tatsuya
× Sakurai, Tatsuya× Tanaka, Miho× 河津, 信一郎× 井上, 昇
WEKO
63
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | The epimastigote form (EMF) of Trypanosoma congolense appears in the late tsetse infective stage. Epimastigotes adhere to the tsetse proboscis, proliferate in this region, and differentiate into mammal-infective metacyclic forms (MCFs). This differentiation is called metacyclogenesis and is indispensable for cyclical transmission of the parasite. Although an in vitro culture method reproducing metacyclogenesis was established several decades ago, few genetic tools have been utilized to elucidate the molecular mechanisms underlying T. congolense metacyclogenesis. In this study, we established a transgene expression system in the EMF of T. congolense IL3000; the EMF was successfully cultured and observed to undergo metacyclogenesis in vitro. The newly constructed expression vector pSAK was designed for integration into the repetitive α-β tubulin locus of the T. congolense genome. pSAK/enhanced green fluorescent protein (eGFP) was transfected into the EMF and procyclic form (PCF), which were cultured in vitro by electroporation. Both EMFs and PCFs expressing eGFP were successfully generated. The eGFP expressing EMFs differentiated into MCFs that continued to express eGFP. The in vitro transgenic EMF generation method is expected to contribute to the elucidation of molecular mechanisms underlying metacyclogenesis. | |||||||||
言語 | en | |||||||||
書誌情報 |
en : Parasitology International 巻 58, 号 1, p. 110-113, 発行日 2009-03 |
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出版者 | ||||||||||
言語 | en | |||||||||
出版者 | Elsevier Ireland | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 13835769 | |||||||||
書誌レコードID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA11112001 | |||||||||
権利 | ||||||||||
権利情報 | Elsevier Ireland | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |