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Genotype and subtype analyses of Cryptosporidium isolates from cattle in Hungary
https://obihiro.repo.nii.ac.jp/records/515
https://obihiro.repo.nii.ac.jp/records/515b6add1c0-5615-4514-b268-607f163d86f8
名前 / ファイル | ライセンス | アクション |
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KaranisP070807.pdf (327.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-08-07 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Genotype and subtype analyses of Cryptosporidium isolates from cattle in Hungary | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cryptosporidium parvum | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cattle | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Genotype | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Subgenotype | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Hungary | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Plutzer, Judit
× Plutzer, Judit× Karanis, Panagiotis |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Seventy-nine faecal samples from calves with diarrhoea were collected on 52 farms from different counties in Hungary to investigate the species, genotypes and subtypes of Cryptosporidium in calves. Oocysts from faecal sample collected from each animal were concentrated using sucrose gradient centrifugation and examined by immunofluorescence assay (IFA). Genomic DNA was extracted from microscopically positive samples and nested PCR was performed to amplify the partial SSU rRNA and GP60 genes of Cryptosporidium that were subsequently digested by SspI, VspI and MboII restriction enzymes to determine the Cryptosporidium species and genotype present. Cryptosporidium parvum was detected in 21 samples while the Cryptosporidium deer-like genotype was found in another sample. The sequence and phylogenetic analysis of 21 isolates of the GP60 PCR products showed that the most common C. parvum subtype is IIaA16G1R1. Interestingly, two isolates were found to contain the C. parvum allele IId in addition to identifying another containing a new C. parvum IIa A18G1R1 subgenotype firstly described in the C. parvum complex. These findings suggest that cattle can be a source of cryptosporidial infections for humans and animals in Hungary. This is the first published description of Cryptosporidium subgenotyping in Hungary. | |||||
言語 | en | |||||
書誌情報 |
en : Veterinary Parasitology 巻 146, 号 3-4, p. 357-362, 発行日 2007-05 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 03044017 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00883655 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Elsevier | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 649 |