WEKO3
アイテム
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Babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen
https://obihiro.repo.nii.ac.jp/records/458
https://obihiro.repo.nii.ac.jp/records/458f65cea0d-7c20-42b6-ad5b-dd4ff09d3c66
名前 / ファイル | ライセンス | アクション |
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n070216-2.pdf (485.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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公開日 | 2007-02-16 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | Babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Babesia gibsoni | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | rhoptry-associated protein | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | enzyme-linked immunosorbent assay | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | diagnosis | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
Zhou, Jinlin
× Zhou, Jinlin× Jia, Honglin× 西川, 義文
WEKO
176
× Fujisaki, Kozo× 玄, 学南
WEKO
295
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抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | A cDNA encoding the rhoptry-associated protein 1 (RAP-1) homologue was obtained by immunoscreening an expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 1740bp. Computer analysis suggested that the sequence contains an open reading frame of 1425bp encoding an expected protein with a molecular weight of 52kDa. Based on the sequence similarity, this putative protein was designated as the B. gibsoni RAP-1 (BgRAP-1). The BgRAP-1 gene was expressed in the Escherichia coli BL21 strain, and the recombinant BgRAP-1 was used as the antigen in the enzyme-linked immunosorbent assay (ELISA). The results can differentiate between the B. gibsoni-infected dog sera and the Babesia canis infected dog sera or the normal dog sera. Furthermore, the antibody response against the recombinant protein was maintained during the chronic stage of infection, indicating that the recombinant BgRAP-1 protein might be a useful diagnostic antigen for the detection of antibodies to B. gibsoni infection in dogs. | |||||||||||||
言語 | en | |||||||||||||
書誌情報 |
en : Veterinary Parasitology 巻 145, 号 1-2, p. 16-20, 発行日 2007 |
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出版者 | ||||||||||||||
言語 | en | |||||||||||||
出版者 | Elsevier | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||
収録物識別子 | 03044017 | |||||||||||||
書誌レコードID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA00883655 | |||||||||||||
権利 | ||||||||||||||
言語 | en | |||||||||||||
権利情報 | Elsevier | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | VoR | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |