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A new protocol to detect multiple foodborne pathogens with PCR dipstick DNA chromatography after a six-hour enrichment culture in a broad-range food pathogen enrichment broth
https://obihiro.repo.nii.ac.jp/records/4469
https://obihiro.repo.nii.ac.jp/records/446937918490-490a-4b54-be94-4c9aab21614e
名前 / ファイル | ライセンス | アクション |
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fulltext (1.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-11-07 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A new protocol to detect multiple foodborne pathogens with PCR dipstick DNA chromatography after a six-hour enrichment culture in a broad-range food pathogen enrichment broth | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hayashi, Masahiro
× Hayashi, Masahiro× Natori, Tatsuya× Kubota-Hayashi, Sayoko× Miyata, Machiko× Ohkusu, Kiyofumi× 川本, 恵子× Kurazono, Hisao× Makino, Souichi× Ezaki, Takayuki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A quick foodborne pathogen screening method after six-hour enrichment culture with a broad-range food pathogen enrichment broth is described. Pathogenic factors of Salmonella enterica, Shigella spp., enteroinvasive Escherichia coli, and enterohemorrhagic E. coli are amplified with a cocktail primer and rapid polymerase chain reaction (PCR), which finishes amplification in 30 min. The PCR amplicon was differentiated with a dipstick DNA chromatography assay in 5-10 min. Starting from a four- to six-hour enrichment culture, this assay was finished within 45 min. Detection sensitivity of this protocol was less than 2.5 CFU/25 g for S. enterica and 3.3 CFU/25 g for enterohemorrhagic E. coli in spiked ground meat experiments. © 2013 Masahiro Hayashi et al. | |||||
言語 | en | |||||
書誌情報 |
en : BioMed Research International 巻 2013, p. 295050, 発行日 2013 |
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出版者 | ||||||
言語 | en | |||||
出版者 | BioMed Central | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1155/2013/295050 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.52/> | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 649 |