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Detection of Trypanosoma cruzi and T. rangeli infections from Rhodnius pallescens bugs by loop-mediated isothermal amplification (LAMP)
https://obihiro.repo.nii.ac.jp/records/4464
https://obihiro.repo.nii.ac.jp/records/4464f8e10c72-6962-4dec-bc26-b1ee7a20bf96
名前 / ファイル | ライセンス | アクション |
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fulltext (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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公開日 | 2019-11-07 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Detection of Trypanosoma cruzi and T. rangeli infections from Rhodnius pallescens bugs by loop-mediated isothermal amplification (LAMP) | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | journal article | |||||||||||
著者 |
Thekisoe, Oriel M. M.
× Thekisoe, Oriel M. M.× Rodriguez, Carol V.× Rivas, Francisco× Coronel-Servian, Andrea M× 福本, 晋也× Sugimoto, Chihiro
WEKO
252
× 河津, 信一郎× 井上, 昇
WEKO
63
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抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | We have developed two loop-mediated isothermal amplification (LAMP) assays for specific detection of Trypanosoma cruzi and Trypanosoma rangeli based on the 18S ribosomal RNA (rRNA) and the small nucleolar RNA (snoRNA) genes, respectively. The detection limit of the assays is 100 fg and 1 pg for T. cruzi and T. rangeli, respectively, with reactions conducted in 60 minutes. The two LAMP assays were used in detection of T. cruzi and T. rangeli infections in comparison with polymerase chain reaction (PCR) for DNA samples extracted from Rhodnius pallescens bugs collected from palm trees in Panama. Out of a total of 52 DNA samples from R. pallescens bugs 17 (33%) and 14 (27%) were T. cruzi-positive by LAMP and PCR, respectively, while, 7 (13%) and 4 (8%) were T. rangeli-positive by LAMP and PCR, respectively. Further evaluation of these LAMP assays is needed, especially with specimens collected from human patients as well as blood kept for transfusion purposes. Copyright © 2010 by The American Society of Tropical Medicine and Hygiene. | |||||||||||
書誌情報 |
en : American Journal of Tropical Medicine and Hygiene 巻 82, 号 5, p. 855-860, 発行日 2010 |
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出版者 | ||||||||||||
言語 | en | |||||||||||
出版者 | American Society of Tropical Medicine and Hygiene | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | PISSN | |||||||||||
収録物識別子 | 00029637 | |||||||||||
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収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AA00521439 | |||||||||||
DOI | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | info:doi/10.4269/ajtmh.2010.09-0533 | |||||||||||
フォーマット | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | application/pdf | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
日本十進分類法 | ||||||||||||
主題Scheme | NDC | |||||||||||
主題 | 649 |