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Improvement of an in vitro drug selection method for generating transgenic Plasmodium berghei parasites
https://obihiro.repo.nii.ac.jp/records/4388
https://obihiro.repo.nii.ac.jp/records/43887ea0e4e3-f260-4524-b116-12dfd2f00b0d
名前 / ファイル | ライセンス | アクション |
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fulltext (3.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-08-08 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Improvement of an in vitro drug selection method for generating transgenic Plasmodium berghei parasites | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Drug selection | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Plasmodium berghei | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Transgenic parasite | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Soga, Akira
× Soga, Akira× Shirozu, Takahiro× Ko-ketsu, Mami× 福本, 晋也 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Reverse genetics approaches have become powerful tools to dissect the biology of malaria parasites. In a previous study, development of an in vitro drug selection method for generating transgenic parasite of Plasmodium berghei was reported. Using this method, two novel and independent selection markers using the P. berghei heat shock protein 70 promoter was previously established. While the approach permits the easy and flexible genetic manipulation of P. berghei, shortcomings include a low variety in promoter options to drive marker gene expression and increased complexity of the selection procedure. In this study, addressing these issues was attempted. Methods: To secure a variety of promoters, the use of a P. berghei elongation factor-1α promoter for marker gene expression was attempted. To simplify the procedure of in vitro selection, the establishment of a two cell-cycle culture method and its application for drug selection were attempted. Results: The P. berghei elongation factor-1α (pbef-1α) promoter, which is commonly used to drive marker gene expression, was successfully applied as an alternative promoter model for marker gene expression, using the parasite's codon-optimized marker sequence. To simplify the in vitro selection method, a two cell-cycle culture method in which the merozoite was released by filtration of the culture containing matured schizont-infected erythrocytes was also developed and successfully applied for drug selection. Conclusion: The pbef-1α promoter was successfully applied in an in vitro selection system. The in vitro selection procedure also could be simplified for practical use using a two cell-cycle culture method. These improvements provide a more versatile platform for the genetic manipulation of P. berghei. © 2019 The Author(s). | |||||
言語 | en | |||||
書誌情報 |
en : Malaria Journal 巻 18, 号 1, p. 215, 発行日 2019 |
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出版者 | ||||||
言語 | en | |||||
出版者 | BioMed Central | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 14752875 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12051150 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1186/s12936-019-2851-6 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 649 |