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TLR2/4 signaling pathway mediates sperm-induced inflammation in bovine endometrial epithelial cells in vitro
https://obihiro.repo.nii.ac.jp/records/4373
https://obihiro.repo.nii.ac.jp/records/437367755af2-8538-4c75-8ef5-63b42ecd5771
名前 / ファイル | ライセンス | アクション |
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fulltext (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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公開日 | 2019-06-13 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | TLR2/4 signaling pathway mediates sperm-induced inflammation in bovine endometrial epithelial cells in vitro | |||||||
言語 | ||||||||
言語 | eng | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
著者 |
Ezz, Mohamed Aboul
× Ezz, Mohamed Aboul× Marey, Mohamed Ali× Elweza, Ahmed Essam× Kawai, Tomoko× Heppelmann, Maike× Pfarrer, Christiane× Balboula, Ahmed Zaky× Motaser, Abdelmonem× Imakawa, Kazuhiko× Zaabel, Samy Moawad× Shimada, Masayuki× Miyamoto, Akio
WEKO
151
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抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | We have recently shown that sperm attachment to bovine endometrial epithelial cells (BEECs) triggers uterine local innate immunity with induction of a pro-inflammatory response in vitro, however details of the mechanism remain unknown. Here, we investigated the involvement of Toll-like receptor 2/4 (TLR2/4) pathway in mediating sperm-BEECs inflammatory process. Immunohistochemistry of the uterine tissue revealed that TLR2 and TLR4 proteins were present in the luminal and glandular epithelia of bovine endometrium. Moreover, BEECs monolayers were treated with TLR2 agonist (Pam; 0, 10, 100, and 1000 ng/ml) or TLR4 agonist (LPS; 0, 0.1, 1, and 10 ng/ml) for 0, 1, 3, or 6 h, followed by evaluating mRNA expression of the pro-inflammatory genes (TNFA, IL-1B, IL-8, and PGES) in BEECs using a real-time PCR. Both Pam and LPS treatments showed a dose-dependent stimulation of mRNA expression of the pro-inflammatory genes. To elucidate the functional role of TLR2/4 in sperm-BEECs interaction, BEECs monolayers were incubated with either TLR2 antagonist or TLR4 antibody for 2 h prior to the co-culture with sperm for 3 h. Importantly, pre-incubation of BEECs with TLR2 antagonist or TLR4 antibody prevented the stimulatory effect of sperm on the transcription of pro-inflammatory genes in BEECs. Furthermore, sperm increased the phosphorylation levels of TLR2/4 downstream targets (p38MAPK and JNK) in BEECs within 1 h of the co-culture. Treatment of BEECs with TLR2 antagonist prior to sperm addition inhibited JNK phosphorylation, while TLR4 antibody inhibited the phosphorylation of both p38MAPK and JNK. In conclusion, the present in vitro findings strongly suggest that bovine endometrial epithelial cells respond to sperm via TLR2/4 signal transduction. © 2019 Ezz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||
言語 | en | |||||||
書誌情報 |
en : PLoS ONE 巻 14, 号 4, p. e0214516, 発行日 2019-04 |
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出版者 | ||||||||
言語 | en | |||||||
出版者 | Public Library of Science | |||||||
ISSN | ||||||||
収録物識別子タイプ | PISSN | |||||||
収録物識別子 | 19326203 | |||||||
DOI | ||||||||
関連タイプ | isIdenticalTo | |||||||
識別子タイプ | DOI | |||||||
関連識別子 | info:doi/10.1371/journal.pone.0214516 | |||||||
権利 | ||||||||
言語 | en | |||||||
権利情報 | This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.0/> | |||||||
フォーマット | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
日本十進分類法 | ||||||||
主題Scheme | NDC | |||||||
主題 | 649 |