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A simple method to detect the tandem repeat of the cyp51A promoter in azole-resistant strains of Aspergillus fumigatus
https://obihiro.repo.nii.ac.jp/records/4296
https://obihiro.repo.nii.ac.jp/records/429630b488fc-ea44-4d56-990b-fe23b25b5103
名前 / ファイル | ライセンス | アクション |
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full text (550.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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公開日 | 2018-11-28 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | A simple method to detect the tandem repeat of the cyp51A promoter in azole-resistant strains of Aspergillus fumigatus | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | real-time PCR | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | cycling probe | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Aspergillus fumigatus | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | tandem repeat | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | azole resistance | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
豊留, 孝仁
× 豊留, 孝仁
WEKO
284
× Hagiwara, Daisuke× Watanabe, Akira× Kamei, Katsuhiko |
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | We designed primers and cycling probes to detect the tandem repeat (TR) of cyp51A promoter in Aspergillus fumigatus. A control-probe was designed to anneal to the outside of the TR region, whereas a TR-probe was designed to anneal to the inside of the TR region. For amplification and probe-hydrolysis detection, the CycleavePCR system was used. Although the difference between Ct values of the wild-type genome for the control-probe and the TR-probe was around -0.1, the difference between Ct values of TR-harboring strains was around 0.7. These data indicate that this is a simple method to detect TR in azole-resistant A. fumigatus. | |||||||||
書誌情報 |
en : Medical mycology 巻 56, 号 8, p. 1042-1044, 発行日 2018-11 |
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出版者 | ||||||||||
出版者 | Oxford University Press | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 14602709 | |||||||||
書誌レコードID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA12422809 | |||||||||
DOI | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | info:doi/10.1093/mmy/myx149 | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | AM | |||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
日本十進分類法 | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 649 |