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Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome
https://obihiro.repo.nii.ac.jp/records/4267
https://obihiro.repo.nii.ac.jp/records/426726d6f01f-b193-470b-bc02-55ae966a02a6
名前 / ファイル | ライセンス | アクション |
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full text (4.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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公開日 | 2018-10-25 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | Urea influences amino acid turnover in bovine cumulus-oocyte complexes, cumulus cells and denuded oocytes, and affects in vitro fertilization outcome | |||||||
言語 | ||||||||
言語 | eng | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
著者 |
Kowsar, Rasoul
× Kowsar, Rasoul× Iranshahi, Vahid Norozian× Sadeghi, Nima× Riasi, Ahmad× Miyamoto, Akio
WEKO
151
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抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | High-protein diets often lead to an increase in urea concentration in follicular fluid of dairy cows, which may reduce oocyte competence. In the present study, maturation media were supplemented with urea (0, 20, 40 mg/dl), and amino acids (AAs) turnover was evaluated in the 24-h spent media of specimens (cell types), bovine cumulus-oocyte complexes (COCs), cumulus cells (CCs), or denuded oocytes (DOs). The main effects of urea and cell type, and their interaction were significant on the individual turnover (expect threonine, glycine, and tyrosine) and total turnover, depletion, and appearance of AAs. The results showed a high level of urea and DOs increased the depletion of all AAs and that of essential and non-AAs, respectively. Sensitivity analysis revealed the highest sensitivity of isoleucine, lysine, and tryptophan to urea, especially in DOs. Principal component analysis (PCA) evaluated the strong correlations between the turnover of: (1) glutamine, aspartic acid or glycine, and developmental competence and fertilization of COCs; (2) serine, isoleucine, valine or glutamic acid, and cleavage rate of DOs; and (3) serine, glutamine, aspartic acid or alanine, and CCs viability. In conclusion, urea significantly changed the turnover of AAs by COCs, CCs and DOs, and reduced the subsequent developmental competence of bovine oocytes. © 2018, The Author(s). | |||||||
書誌情報 |
en : Scientific Reports 巻 8, 号 1, p. 12191, 発行日 2018-12-01 |
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出版者 | ||||||||
出版者 | Nature Publishing Group | |||||||
ISSN | ||||||||
収録物識別子タイプ | ISSN | |||||||
収録物識別子 | 20452322 | |||||||
DOI | ||||||||
関連タイプ | isIdenticalTo | |||||||
識別子タイプ | DOI | |||||||
関連識別子 | info:doi/10.1038/s41598-018-30774-2 | |||||||
権利 | ||||||||
権利情報 | This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.0/> | |||||||
フォーマット | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
日本十進分類法 | ||||||||
主題Scheme | NDC | |||||||
主題 | 645 |