WEKO3
アイテム
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Novel Species Specific Antigens of Trypanosoma congolense and Their Different Localization among Life-Cycle Stages
https://obihiro.repo.nii.ac.jp/records/4242
https://obihiro.repo.nii.ac.jp/records/4242afe0cf6a-8bb3-4377-99ac-9145f4a3e030
名前 / ファイル | ライセンス | アクション |
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full text (398.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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公開日 | 2018-08-27 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Novel Species Specific Antigens of Trypanosoma congolense and Their Different Localization among Life-Cycle Stages | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Confocal laser scanning microscope | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Life cycle | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Monoclonal antibody | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Species specific antigen | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Trypanosoma congolense | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
井上, 昇
× 井上, 昇
WEKO
63
× Lluz, Archie Tafalla× Mori, Taiki× Nagasawa, Hideyuki× Fujisaki, Kozo× Mikami, Takeshi |
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | Seven monoclonal antibodies (mAbs) were raised against Trypanosoma congolense procyclic form (PCF). Localization of the antigens recognized by the mAbs was determined in bloodstream form (BSF), PCF, epimastigote form (EMF) and metacyclic form (MCF) by confocal laser scanning microscopy (CLSM). Two mAbs (10F9 and 20H12) showed different fluorescent patterns among different life-cycle stages of the parasite. The 10F9 recognized a 76 kDa antigen of all life-cycle stages of the parasite and the antigen localization corresponded with that of a mitochondrion. While the 20H12 recognized 119 and 122 kDa antigens of all the life-cycle stages and the antigen localization corresponded with a flagellum in BSF and MCF, tip of a flagellum in PCF, and part of cytoplasm in EMF. Moreover, the 20H12 did not react to T. brucei gambiense, T. b. rhodesiense and T. evansi antigens in both CLSM and immunoblotting. Therefore, the antigens recognized by the 20H12 seem to be T. congolense specific. Although, further studies will be required for a full characterization of the T. congolense specific 119 and 122 kDa antigens, the mAb 20H12 and the specific antigens may be useful in not only establishment of 7. congolense specific diagnosis methods but also studies on molecular mechanisms regulating differentiation of the parasite during life-cycle. | |||||||||
言語 | en | |||||||||
書誌情報 |
en : Journal of Veterinary Medical Science 巻 62, 号 10, p. 1041-1045, 発行日 2000 |
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出版者 | ||||||||||
言語 | en | |||||||||
出版者 | Japanese Society of Veterinary Science | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 09167250 | |||||||||
書誌レコードID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA10796138 | |||||||||
DOI | ||||||||||
関連タイプ | isIdenticalTo | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | info:doi/10.1292/jvms.62.1041 | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
日本十進分類法 | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 649 |