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Study on endocytosis and hemoglobin uptake in different developmental stages of Trypanosoma congolense IL3000 strain
https://obihiro.repo.nii.ac.jp/records/4153
https://obihiro.repo.nii.ac.jp/records/415363d0403f-8b3d-4a6a-9da5-99ba1cb038de
名前 / ファイル | ライセンス | アクション |
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fulltext (296.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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公開日 | 2018-02-22 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | Study on endocytosis and hemoglobin uptake in different developmental stages of Trypanosoma congolense IL3000 strain | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | endocytosis | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | haptoglobin-hemoglobin receptor | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Trypanosoma congolense | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
Munsimbwe, L.
× Munsimbwe, L.× 菅沼, 啓輔
WEKO
357
× Yamasaki, S.× Mochabo, K. M.× 河津, 信一郎× 井上, 昇
WEKO
63
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抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | The study aimed to show endocytosis and hemoglobin uptake in Trypanosoma congolense. All the different developmental stages of T. congolense, IL3000 strain were cultured with dextran Alexa Fluor® 568 10,000 MW, dextran fluorescein 70,000 MW and hemoglobin labeled with Alexa Fluor® 488. The trypanosomes were also incubated in Hoechst® 33342 and observed by confocal laser scanning microscopy. The results showed that non-specific endocytosis was up-regulated in metacyclic form (MCF) and bloodstream form (BSF) whereas receptor-mediated hemoglobin endocytosis was highly activated only in the insect stage epimastigote form (EMF). This might indicate that the rapid uptake of surface-bound molecules in MCF and BSF may be important in the survival of the trypanosomes in the mammalian host. However, there was no observed non-specific endocytosis in the two insect stages procyclic form (PCF) and EMF, which might suggest that these stages totally depend on receptor-mediated endocytosis. Furthermore, the results showed uptake of hemoglobin in EMF but not in other stages. This might explain as to why a haptoglobinhemoglobin receptor (TcHpHbR) has been specifically expressed in EMF stage of T. congolense. Therefore, TcHpHbR mediated hemoglobin requirement of the insect stage is probably higher than other stages because of their habitat and fully activated mitochondrial metabolism. | |||||||||||||
言語 | en | |||||||||||||
書誌情報 |
en : Journal of Protozoology Research 巻 23, 号 1-2, p. 14-20, 発行日 2013-12 |
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出版者 | ||||||||||||||
言語 | en | |||||||||||||
出版者 | National Research Center for Protozoan Diseases | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||
収録物識別子 | 09174427 | |||||||||||||
書誌レコードID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA10824856 | |||||||||||||
権利 | ||||||||||||||
言語 | en | |||||||||||||
権利情報 | National Research Center for Protozoan Diseases | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | VoR | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
日本十進分類法 | ||||||||||||||
主題Scheme | NDC | |||||||||||||
主題 | 649 |