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低温性通性雪腐菌核病菌Sclerotinia trifoliorum のアルファルファ培養において産生されるポリガラクツロナーゼ活性
https://obihiro.repo.nii.ac.jp/records/1840
https://obihiro.repo.nii.ac.jp/records/18402178b6a2-d6cf-4ea8-b264-c44254dcf37f
名前 / ファイル | ライセンス | アクション |
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w061121-1.pdf (1.2 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-11-21 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 低温性通性雪腐菌核病菌Sclerotinia trifoliorum のアルファルファ培養において産生されるポリガラクツロナーゼ活性 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sclerotinia trifoliorum | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
主題 | 低温菌 | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
主題 | ポリガラクツロナーゼ | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
主題 | 低温適応 | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
主題 | 細胞壁分解酵素 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sclerotinia trifoliorum | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | psychrotroph | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | polygalacturonase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cold adaptaition | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cell wall degrading enzyme | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | Polygalacturonase activity produced by alfalfa culture of the psychrotrophic facultative snow mold Sclerotinia trifoliorum | |||||
言語 | en | |||||
著者 |
渡辺, 剛志
× 渡辺, 剛志× 波川, 啓土× 斉藤, 泉× 高澤, 俊英 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 低温性通性雪腐菌核病菌Sclerotinia trifoliorum の低温適応現象を解明するために, 5℃及び20℃で産生するポリガラクツロナーゼ(PGase) 活性の性質を調べた。S. trifoliorum のアルファルファ培地での培養は5℃では160日間(最適培養日数), 20℃では45日間(最適培養日数) 行った。5℃及び20℃培養粗抽出液のPGase 活性は, それぞれ30.8±0.4 U/mL (抽出体積462mL, Total activity 14 200U), 36.5±0.9 U/mL (抽出体積454mL, Total activity 16 600U) であり, 低温においてもPGase 産生量が著しく低下しないことがわかった。これら粗抽出液をBio-Gel P-6 DG ゲル濾過クロマトグラフィーによる脱塩操作後に, 5℃及び20℃培養脱塩画分のPGase 活性の性質を調べた。 温度依存性については, 最適温度は5℃及び20℃培養PGase 活性ともに50℃であり, 50℃での活性に対する各温度での相対活性によって両者を較べると, それらの曲線は両者でほぼ一致した。温度安定性については, 残存活性で比較すると, 5℃培養PGase 活性は15℃~35℃において僅かであるが20℃培養のものにくらべて不安定性を示した。一方, 45~55℃では若干の安定性を示した。pH 依存性については, 5℃培養PGase 活性の最適pH は4.5であり, 20℃培養は4.0であった。従って, これらの結果は5℃及び20℃培養によって産生された各々のPGase アイソザイムが異なることを示した。 |
|||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In order to clarify cold adaptation phenomenon of the psychrotrophic facultative snow mold Sclerotinia trifoliorum, the properties of polygalacturonase (PGase) activity produced in 5℃-and 20℃-culture were examined in the crude extracts. S. trifoliorum was cultured in the alfalfa medium at 5℃ for 160 days and at 20℃ for 45 days. The PGase activities in the crude extracts of the 5℃- and 20℃-culture were 30.8±0.4 U/mL (Extracted volume, 462 mL; Total activity, 14 200 U), 36.5±0.9U/mL (Extracted volume, 454 mL; Total activity, 16 600 U), respectively. It was shown that PGase production did not remarkably decrease in the low-temperature environment. After desalting of these crude extracts by the Bio-Gel P-6 DG gel filtration chromatography, the properties of PGase activities of the 5℃-and 20℃-culture desalting fractions were examined. As to the temperature dependence, the optimum temperature for the enzyme reaction at pH 4.5 was 50℃ for both 5℃-and 20℃-culture. Both cultures were compared by the relative activity against the activity at 50℃, and both dependency-curves almost agreed within an experimental error. In the thermostability, compared with the 20℃-culture by remaining activity, the 5℃-culture PGase activity showed slightly more instability at 15℃-35℃, but a little more stability at 45℃-55℃. For the pH dependence, the optimum pH of 5℃-culture PGase activity was 4.5, and 20℃-culture was 4.0. Therefore, this fact shows that PGase isoenzymes produced by 5℃-and 20℃-culture were different from each other. |
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言語 | en | |||||
書誌情報 |
ja : 帯広畜産大学学術研究報告 en : Research bulletin of Obihiro University 巻 25, p. 15-21, 発行日 2004-08 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 帯広畜産大学 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 13485261 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11877585 | |||||
権利 | ||||||
言語 | ja | |||||
権利情報 | 帯広畜産大学 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
言語 | ja | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |