WEKO3
アイテム
Rapid Detection of Plastmodium by a New ‘Thick Smear’ Method using Transmission Fluorescence Microscopy : Direct Staining with Acridine Orange
https://obihiro.repo.nii.ac.jp/records/1729
https://obihiro.repo.nii.ac.jp/records/1729ad7f2c36-c1a2-4426-bb3b-95d910d8f1b1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Prot.Vol.1-(4).pdf (2.5 MB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-12-21 | |||||
| タイトル | ||||||
| タイトル | Rapid Detection of Plastmodium by a New ‘Thick Smear’ Method using Transmission Fluorescence Microscopy : Direct Staining with Acridine Orange | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | acridine orange | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | fluorochrome staining | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | interference filter | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Plasmodium | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | thick smear | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Kawamoto, Fumihiko
× Kawamoto, Fumihiko |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A new staining technique of 'thick smears' was developed tor diagnosis of malaria using transmission fluorescence microscopy. Plasmodium falciparum cultured in vitro was used as a model. The infected blood was mixed 1 : 1 -1 :2 with acridine orange (AO) solution (final concentrations of 50-lOOu.g/ml in 0.01 M Tris-HCI buffer or in PBS, pH 7.0-7.5). Immediately or several minutes later, haemolyzed or non-haemolyzed 'thick smears' were observed directly by fluorescence microscopy using an interference filter specially designed for excitation of AO or commercially available, three (interference-, glass- and triacetyl cellulose film-) types of B-excitation filters with halogen- or daylight-illuminated microscopes. All of these filters were capable of detecting the parasites rapidly in these wet mounts at a magnification of x200 using standard lenses. Among them, the interferencetype filters having higher transmission efficiencies gave the best results. Film filter system, which was the cheapest, could also be used, especially in combination with daylightilluminated microscopes, although it was necessary to be exposed accurately to the direct rays of the sun. These results strongly suggest that transmission fluorescence microscopy using any B-excitation filter in light microscopes may be a useful economic system for rapid diagnosis of malaria. |
|||||
| 言語 | en | |||||
| 書誌情報 |
en : The journal of protozoology research 巻 1, p. 27-34, 発行日 1991-10 |
|||||
| 出版者 | ||||||
| 出版者 | Research Center for Protozoan Molecular Immunology | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 09174427 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10824856 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | Research Center for Protozoan Molecular Immunology | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 言語 | en | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 649 | |||||
