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The Influence of the regulation of Toxoplasma gondii TgMIC2 transgene on host cell infection
名前 / ファイル | ライセンス | アクション |
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B090202-5.pdf (476.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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公開日 | 2009-02-02 | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | en | |||||||||||||||||
タイトル | The Influence of the regulation of Toxoplasma gondii TgMIC2 transgene on host cell infection | |||||||||||||||||
言語 | ||||||||||||||||||
言語 | eng | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | Toxoplasma gondii | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | TgMIC2 | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | over-expression | |||||||||||||||||
資源タイプ | ||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
資源タイプ | journal article | |||||||||||||||||
著者 |
Buates, S.
× Buates, S.× 玄, 学南
WEKO
295
× Igarashi, Makoto
WEKO
324
× Sugimoto, Chihiro
WEKO
252
× 井上, 昇
WEKO
63
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抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | Toxoplasma gondii microneme protein 2 (TgMIC2), an apically stored adhesin, was shown to be a key participant involved in the initial attachment and invasion to a host cell. In this study, we had established the clonal line of T. gondii tachyzoites which over-expressed TgMIC2 using the tetracycline repressor (TetR)-based inducible gene expression system. The TgMIC2 over-expression significantly reduced tachyzoite propagation in vitro (p < 0.002) and significantly reduced the parasite virulence in mice (p = 0.002). The over-expression of exogenous TgMIC2 caused the increase of mRNA expression level of endogenous TgMIC2. Wild type parasites mainly expressed TgMIC2115 protein, the microneme store and the surface form of TgMIC2. Over-expression of TgMIC2 induced production of TgMIC2110, the cell surface form of TgMIC2 produced by N-terminal proteolytic processing of TgMIC2115. Some of tachyzoites became spherical shape having disordered apical complex after TgMIC2 over-expression. TgMIC2 over-expression did not effect mRNA expression level of TgMIC2-associated protein (TgM2AP), but appeared to cause the aberrance of TgM2AP protein. In conclusion, our results reveal the novel findings on the importance of the regulation of TgMIC2 transgene expression on post-translational modification of TgMIC2 and TgM2AP, and parasite’s morphology and propagation, with all together impacting the outcome of infection. | |||||||||||||||||
言語 | en | |||||||||||||||||
書誌情報 |
en : The journal of protozoology research 巻 18, 号 2, p. 79-95, 発行日 2008-12 |
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出版者 | ||||||||||||||||||
言語 | en | |||||||||||||||||
出版者 | National Research Center for Protozoan Diseases | |||||||||||||||||
ISSN | ||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||
収録物識別子 | 09174427 | |||||||||||||||||
書誌レコードID | ||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||
収録物識別子 | AA10824856 | |||||||||||||||||
権利 | ||||||||||||||||||
言語 | en | |||||||||||||||||
権利情報 | National Research Center for Protozoan Diseases | |||||||||||||||||
フォーマット | ||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||
内容記述 | application/pdf | |||||||||||||||||
著者版フラグ | ||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
日本十進分類法 | ||||||||||||||||||
主題Scheme | NDC | |||||||||||||||||
主題 | 649 |