WEKO3
アイテム
{"_buckets": {"deposit": "52980b72-2937-4c99-95e9-09ff7b45769b"}, "_deposit": {"created_by": 12, "id": "145", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "145"}, "status": "published"}, "_oai": {"id": "oai:obihiro.repo.nii.ac.jp:00000145", "sets": ["5"]}, "author_link": ["571", "334", "168", "151"], "item_2_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2000", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "114", "bibliographicPageStart": "109", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "Journal of Reproduction and Development", "bibliographic_titleLang": "en"}]}]}, "item_2_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The presence of gonadotropin-releasing hormone (GnRH)-like protein has been previously demonstrated in the bovine follicle. This GnRH-like protein was purified and concluded to be histone H2A. However, neither GnRH peptide nor specific GnRH-immunoreactivity (GnRH-IR) has been demonstrated in the bovine follicle so far. Thus, this study focused on the detection of specific GnRH-IR using the second-antibody enzyme immunoassay in isolated bovine mature follicles, and on an examination of the direct effect of luteinizing hormone (LH), endothelin-1 (ET-1), and cytokines on the GnRH-IR using an in vitro microdialysis system (MDS). We further examined a cross-reactivity of the GnRH antibody with bovine histone H2A. GnRH-IR was detected in microdialyzed perfusate from isolated bovine mature follicles at 4.40 ± 0.35 pg/ml (mean ± SEM). Bovine histone H2A showed no GnRH-IR at all. Heat treatment of the extract (100 C for 10 min) did not affect the GnRH-IR. Single infusion of LH, ET-1, or cytokines into the MDS did not affect the GnRH-IR. However, infusion of ET-1 after LH exposure increased the GnRH-IR. These results demonstrate the presence of specific GnRH-IR, that is different from histone H2A, in microdialyzed perfusate of isolated bovine mature follicles in vitro, suggesting that the GnRH-IR may reflect a role of GnRH-like peptide or some peptide structurally similar to GnRH in the local regulation of mature follicles.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_2_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Japanese Society of Animal Reproduction", "subitem_publisher_language": "en"}]}, "item_2_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1262/jrd.46.109", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Japanese Society of Animal Reproduction", "subitem_rights_language": "en"}]}, "item_2_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10936678", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "09168818", "subitem_source_identifier_type": "PISSN"}]}, "item_2_text_25": {"attribute_name": "文献提供者", "attribute_value_mlt": [{"subitem_text_value": "宮本明夫(akiomiya@obihiro.ac.jp)"}]}, "item_2_textarea_24": {"attribute_name": "作業記録", "attribute_value_mlt": [{"subitem_textarea_value": "Submitted by リポジトリ管理者 repo (repo@obihiro.ac.jp) on 2011-06-27T07:14:28Z\nNo. of bitstreams: 1\nimmuno.pdf: 247607 bytes, checksum: 077f1bdcbd9e04711ea3874dd44732fc (MD5)"}, {"subitem_textarea_value": "Made available in DSpace on 2011-06-27T07:14:28Z (GMT). No. of bitstreams: 1\nimmuno.pdf: 247607 bytes, checksum: 077f1bdcbd9e04711ea3874dd44732fc (MD5)\n Previous issue date: 2000"}]}, "item_2_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ozawa, Takahiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "571", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Acosta, Tomas J.", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "334", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000002065458", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000002065458"}, {"nameIdentifier": "80379718", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80379718"}, {"nameIdentifier": "read0118629", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0118629"}]}, {"creatorNames": [{"creatorName": "Nakamura, Tadashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "168", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000070271749", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000070271749"}, {"nameIdentifier": "70271749", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70271749"}]}, {"creatorNames": [{"creatorName": "Miyamoto, Akio", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "151", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000010192767", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000010192767"}, {"nameIdentifier": "10192767", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=10192767"}, {"nameIdentifier": "read0167498", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "https://researchmap.jp/read0167498"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-01-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "immuno.pdf", "filesize": [{"value": "247.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 247600.0, "url": {"label": "immuno.pdf", "url": "https://obihiro.repo.nii.ac.jp/record/145/files/immuno.pdf"}, "version_id": "abe76873-552d-4ab0-83e8-0c1aaa7da274"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "GnRH", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Immunoreactivity", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Microdialysis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Follicle", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cow", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Immunoreactivity for Gonadotropin-Releasing Hormone in Microdialyzed Perfusates of Bovine Mature Follicles In Vitro", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Immunoreactivity for Gonadotropin-Releasing Hormone in Microdialyzed Perfusates of Bovine Mature Follicles In Vitro", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "12", "path": ["5"], "permalink_uri": "https://obihiro.repo.nii.ac.jp/records/145", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2011-06-27"}, "publish_date": "2011-06-27", "publish_status": "0", "recid": "145", "relation": {}, "relation_version_is_last": true, "title": ["Immunoreactivity for Gonadotropin-Releasing Hormone in Microdialyzed Perfusates of Bovine Mature Follicles In Vitro"], "weko_shared_id": -1}
Immunoreactivity for Gonadotropin-Releasing Hormone in Microdialyzed Perfusates of Bovine Mature Follicles In Vitro
https://obihiro.repo.nii.ac.jp/records/145
https://obihiro.repo.nii.ac.jp/records/14592129d98-b19a-4145-ab34-f848733c06be
名前 / ファイル | ライセンス | アクション |
---|---|---|
immuno.pdf (247.6 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2011-06-27 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Immunoreactivity for Gonadotropin-Releasing Hormone in Microdialyzed Perfusates of Bovine Mature Follicles In Vitro | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | GnRH | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Immunoreactivity | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Microdialysis | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Follicle | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Cow | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Ozawa, Takahiro
× Ozawa, Takahiro× Acosta, Tomas J.
WEKO
334
× Nakamura, Tadashi× Miyamoto, Akio
WEKO
151
|
|||||||||
抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | The presence of gonadotropin-releasing hormone (GnRH)-like protein has been previously demonstrated in the bovine follicle. This GnRH-like protein was purified and concluded to be histone H2A. However, neither GnRH peptide nor specific GnRH-immunoreactivity (GnRH-IR) has been demonstrated in the bovine follicle so far. Thus, this study focused on the detection of specific GnRH-IR using the second-antibody enzyme immunoassay in isolated bovine mature follicles, and on an examination of the direct effect of luteinizing hormone (LH), endothelin-1 (ET-1), and cytokines on the GnRH-IR using an in vitro microdialysis system (MDS). We further examined a cross-reactivity of the GnRH antibody with bovine histone H2A. GnRH-IR was detected in microdialyzed perfusate from isolated bovine mature follicles at 4.40 ± 0.35 pg/ml (mean ± SEM). Bovine histone H2A showed no GnRH-IR at all. Heat treatment of the extract (100 C for 10 min) did not affect the GnRH-IR. Single infusion of LH, ET-1, or cytokines into the MDS did not affect the GnRH-IR. However, infusion of ET-1 after LH exposure increased the GnRH-IR. These results demonstrate the presence of specific GnRH-IR, that is different from histone H2A, in microdialyzed perfusate of isolated bovine mature follicles in vitro, suggesting that the GnRH-IR may reflect a role of GnRH-like peptide or some peptide structurally similar to GnRH in the local regulation of mature follicles. | |||||||||
言語 | en | |||||||||
書誌情報 |
en : Journal of Reproduction and Development 巻 46, 号 2, p. 109-114, 発行日 2000 |
|||||||||
出版者 | ||||||||||
言語 | en | |||||||||
出版者 | Japanese Society of Animal Reproduction | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 09168818 | |||||||||
書誌レコードID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA10936678 | |||||||||
DOI | ||||||||||
関連タイプ | isIdenticalTo | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | info:doi/10.1262/jrd.46.109 | |||||||||
権利 | ||||||||||
言語 | en | |||||||||
権利情報 | Japanese Society of Animal Reproduction | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |