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Evaluation of usefulness of a commercial agarose gel electrophoresis kit (QuickGel SP) for bovine serum protein electrophoresis
https://obihiro.repo.nii.ac.jp/records/1287
https://obihiro.repo.nii.ac.jp/records/1287a89d74f3-f480-4cdd-a490-e2dcbdb4d38e
名前 / ファイル | ライセンス | アクション |
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pjvs-2017-0064.pdf (1.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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公開日 | 2018-01-19 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | Evaluation of usefulness of a commercial agarose gel electrophoresis kit (QuickGel SP) for bovine serum protein electrophoresis | |||||||
言語 | ||||||||
言語 | eng | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | agarose gel electrophoresis | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | bovine serum protein | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | cellulose acetate electrophoresis | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | QuickGel SP | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
著者 |
Okatsu, Y.
× Okatsu, Y.× Yamagishi, N.
WEKO
300
× Hatate, K.× Devkota, B. |
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抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | The aim of this study was to show the usefulness of a commercial agarose gel electrophoresis (AGE) kit (QuickGel SP) for separating bovine serum protein fractions in comparison with conventional cellulose acetate electrophoresis (CAE). Serum protein bands were verified using five reference reagents corresponding to albumin and α1-, β1-, β2-, and γ-globulins. AGE clearly revealed six separated fractions of albumin and α1-, α2-, β1-, β2-, and γ-globulin fractions in 100% and 77.8% in serum samples of dairy cows from the healthy (n=27) and diseased groups (n=27), respectively. The α1- and α2-globulins were not separated by CAE in 14.8% and 96.3% of the samples from the healthy and diseased groups, respectively, whereas β2- and γ-globulin were not separated by CAE in 96.3% and 100% of the samples from the healthy and diseased groups, respectively. More than 94% of the points for the α-globulin fractions (α1- and α2-globulins), the β-γ-globulin fractions (β1-, β2-, and γ-globulins), and the albumin/globulin ratio between AGE and CAE were within agreement on the Bland-Altman plots. However, the mean biases were not near zero in the albumin and β-γ-globulin fractions. These results suggest that the high-resolution commercial AGE kit can be utilized to separate bovine serum protein fractions. (c) Polish Academy of Sciences, Committee of Veterinary Sciences &University of Warmia and Mazury in Olsztyn 2017. | |||||||
言語 | en | |||||||
書誌情報 |
en : Polish Journal of Veterinary Sciences 巻 20, 号 3, p. 527-534, 発行日 2017-09 |
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出版者 | ||||||||
言語 | en | |||||||
出版者 | Polish Academy of Sciences | |||||||
ISSN | ||||||||
収録物識別子タイプ | PISSN | |||||||
収録物識別子 | 15051773 | |||||||
書誌レコードID | ||||||||
収録物識別子タイプ | NCID | |||||||
収録物識別子 | AA12262757 | |||||||
DOI | ||||||||
関連タイプ | isIdenticalTo | |||||||
識別子タイプ | DOI | |||||||
関連識別子 | info:doi/10.1515/pjvs-2017-0064 | |||||||
権利 | ||||||||
言語 | en | |||||||
権利情報 | This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0 | |||||||
フォーマット | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
日本十進分類法 | ||||||||
主題Scheme | NDC | |||||||
主題 | 645 |