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Evaluation of novel oocyst wall protein candidates of Toxoplasma gondii
https://obihiro.repo.nii.ac.jp/records/1247
https://obihiro.repo.nii.ac.jp/records/1247bfd74ed0-e829-4cbe-88a9-6bb5c20436b2
名前 / ファイル | ライセンス | アクション |
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1-s2.0-S1383576917301848-main.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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公開日 | 2017-10-18 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | Evaluation of novel oocyst wall protein candidates of Toxoplasma gondii | |||||||
言語 | ||||||||
言語 | eng | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | T. gondii oocyst wall protein | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Toxoplasma gondii | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
著者 |
Salman, Doaa
× Salman, Doaa× Okuda, Liria Hiromi× Ueno, Akio× Dautu, George× Zhang, Feifei× Igarashi, Makoto
WEKO
324
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抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | Oocyst stage of Toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. Thus, it is considered the key for transmission of T. gondii. Analysis of oocyst wall composition is mandatory therefore; the aim of this study was to identify novel T. gondii oocyst wall proteins and test their use in detection of these oocysts in environmental samples. Five candidates of novel T. gondii oocyst wall proteins (TgOWPs) were identified and named TgOWP8 through TgOWP12. Recombinant protein of TgOWP8 was expressed in E. coli using glutathione S-transferase as fusion protein. Polyclonal antibody was produced and validated by indirect immunofluorescence antibody assay (IFA). For detection by IFA, we used different methods for fixation and permeabilization of oocysts to improve the antigen-antibody detection. Specificity to wall of T. gondii oocyst was confirmed and revealed absence of cross reactivity with bradyzoite cyst wall and tachyzoites. Although some TgOWPs were identified previously, our study represents a continuation of molecular investigations of oocyst wall proteins as an essential structure for the longevity and infectivity of this stage and also provided new trial to improve T. gondii oocysts detection. c 2017 The Authors | |||||||
言語 | en | |||||||
書誌情報 |
en : Parasitology International 巻 66, 号 5, p. 643-651, 発行日 2017 |
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出版者 | ||||||||
言語 | en | |||||||
出版者 | Elsevier Ireland | |||||||
ISSN | ||||||||
収録物識別子タイプ | PISSN | |||||||
収録物識別子 | 13835769 | |||||||
書誌レコードID | ||||||||
収録物識別子タイプ | NCID | |||||||
収録物識別子 | AA11112001 | |||||||
DOI | ||||||||
関連タイプ | isIdenticalTo | |||||||
識別子タイプ | DOI | |||||||
関連識別子 | info:doi/10.1016/j.parint.2017.05.009 | |||||||
権利 | ||||||||
言語 | en | |||||||
権利情報 | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). | |||||||
フォーマット | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
日本十進分類法 | ||||||||
主題Scheme | NDC | |||||||
主題 | 491 |